一个水稻黄叶突变体的叶绿素合成关键基因的表达分析

Expression Analysis of Key Genes of Chlorophyll Synthesis in A Yellow Leaves Mutant of Oryza sativa

为了研究水稻黄叶突变基因与叶绿素合成途径的关系及突变基因的作用机理,本研究以野生型作对照,结合半定量与实时定量PCR技术,对突变体茎和叶组织中叶绿素合成关键基因的RNA表达水平进行了分析。结果显示,在茎组织中,研究选取的8个基因在突变体和野生型中的表达均没有明显差别。而在叶组织中,突变体的YGL1和CHLI两个基因的表达量约为野生型的1.8倍,表达明显上调;CHLD基因的表达量约为野生型的0.6倍,表达明显下调;其余5个基因的表达没有明显差别。研究表明,突变基因能够调控叶绿素合成关键基因YGL1、CHLD和CHLI的表达,从而影响叶组织中叶绿素的合成,这为进一步研究突变基因对叶绿素合成的调控模式及其作用机理奠定了基础。

The aims are to study the relationship between rice yellow leaf mutant gene and chlorophyll synthesis,and the mechanism of mutant genes.In this study,the wild type was taken as the control,the RNA expression of key genes of chlorophyll biosynthesis in stems and leaves of mutants were analyzed by semiquantitative and real-time quantitative PCR.The results showed that there was no significant difference in the expression of 8 genes of stems in the wild type and the mutants.In the leaves,the expression of YGL1 and CHLI in the mutant was significantly up-regulated,about 1.8 times as that in wild type.The expression of CHLD gene in the mutant was about 0.6 times as that in wild type,was significantly down-regulated.The expression of the other 5 genes was not significantly different between the mutants and the wild type.The results indicated that the mutant gene could regulate the expression of YGL1,CHLD and CHLI genes and further affect the synthesis of chlorophyll in leaves.The study laid a foundation for understanding the role of mutant gene in the regulation of chlorophyll synthesis and its functional mechanism.