经介入途径多疗法、多基因联合治疗肝癌的可行性分析

Feasibility Analysis of Combined Therapy and Multiple Gene Therapy for Hepatocellular Carcinoma

目的:分析探讨经介入途径多疗法、多基因联合治疗肝癌的可行性。方法:选取2013年11月-2016年11月120只大白兔建立肝癌模型,按照随机数字表法分为五组,每组24只。第一组为单纯超液化碘油栓塞组,第二组为超液化碘油+P53(质粒-脂质体复合物)组,第三组为超液化碘油+TK/CD(浓缩的TK-CD逆转录病毒上清),第四组为超液化碘油+P53+TK/CD组,第五组仅进行生理盐水治疗(对照组)。对五组大白兔进行常规的病理检查和生存期观测。结果:各治疗组肿瘤体积增长率均较对照组明显下降,其中第四组肿瘤增长率抑制最为有效,差异均有统计学意义(P<0.05);对照组瘤兔介入术后20~40 d相继全部死亡,治疗组在观察期内自然死亡30只(31.3%),第一组12只(50.0%),第二组9只(37.5%),第三组7只(29.2%),第四组2只(8.3%),五组瘤兔生存情况比较差异均有统计学意义(P<0.05)。结论:介入基因疗法能够克服介入治疗的副作用,有效地抑制肿瘤的生长,延长瘤兔的生存时间。

Objective： To investigate the feasibility of combined therapy and multiple gene therapy in the treatment of liver cancer.Method： A total of 120 rabbits from November 2013 to November 2016 were selected to establish the model of liver cancer, and they were randomly divided into 5 groups according to random number table method, 24 cases in each group.The first group was given pure lipiodol embolization group, the second group was given lipiodol＋P53 （ plasmid liposome complex ） group, the third group was lipiodol＋TK/CD （ TK-CD retrovirus supernatant concentration ）, the fourth group was given lipiodol＋P53＋TK/CD group, the fifth group only saline the treatment （ control group ） .Five groups were examined by routine pathology and their lifetime were observed.Result： Compared with the control group, the tumor volume growth rate of the other groups decreased significantly, the fourth group was the most effective, the differences were statistically significant （P〈0.05） . Rabbits of the control group had all died after PCI 20-40 days, natural death of the other grouPs inthe observation period was 30 cases （ 31.3% ）, the first group was 12 cases （ 50.0% ）, the second group 9 cases （ 37.5% ）, the third group was 7 cases （ 29.2% ）, the fourth group was 2 cases （ 8.3% ）, the differences were statistically significant （ P〈0.05 ） .Conclusion： Interventional gene therapy can overcome the side effects of interventional therapy, effectively inhibit the grola of tumor and prolong the survival time of tumor rabbits.