知母多糖治疗糖尿病大鼠

Polysaccharide from Anemarrhena asphodeloides treats diabetic rats

目的探讨知母多糖对糖尿病大鼠的治疗作用及潜在作用机制。方法腹腔注射STZ(60 mg/kg)法建立糖尿病大鼠模型,60只大鼠分为正常组、模型组、格列苯脲组(25 mg/kg)和知母多糖组(50、100、200 mg/kg),灌胃给药大鼠,每天1次。28 d后,检测大鼠体质量、葡萄糖耐量、空腹血糖(FBG)、空腹胰岛素(FINS)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、丙二醛(MDA)、胰岛素受体底物1(IRS1)、磷酸化的胰岛素受体底物1(Phospho-IRS1)及葡萄糖转运蛋白4(Glut4)。结果知母多糖可以增加大鼠体质量,并改善对葡萄糖的耐受能力;降低FBG水平,提高FINS水平;也可以降低模型大鼠血清IL-6、TNF-α水平及肝组织MDA含有量,提高肝组织CAT、SOD活性。同时,知母多糖还可以减少肝组织Phospho-IRS1表达,增加Glut4表达。结论知母多糖具有抗糖尿病作用,其机理包括抗炎、抗氧化、减少Phospho-IRS1的表达及增加Glut4的表达。

AIM To explore the therapeutic effects of polysaccharide from Anemarrhena asphodeloides Bunge（ PAA） on diabetic rats and potential mechanism of action. METHODS The rat model for diabetes was established by intraperitoneal injection of STZ（ 60 mg/kg）,and sixty rats were assigned to control-,model-,glibenclamide-（ 25 mg/kg） groups and three groups of PAA-（ 50,100,200 mg/kg）. Drugs were intragastrically administrated to rats once a day for 28 days. The rat body weight,glucose tolerance,fasting blood glucose（ FBG）,fasting insulin（ FINS）,IL-6,TNF-α,CAT,SOD,MDA,insulin receptor substrate（ IRS1）,Phospho-IRS1 and glucose transporter protein 4（ Glut4） were tested. RESULTS PAA could increase rat body weight and FINS level,reduce FBG level,and improve the glucose tolerance. The levels of IL-6 and TNF-α in serum,and MDA content in hepatic tissue were decreased,and the activities of CAT and SOD in hepatic tissue were increased.Meanwhile,PAA could also reduce the Phospho-IRS1 expression,and increase the Glut4 expression in hepatic tissue. CONCLUSION PAA has an anti-diabetic effect,whose mechanism is involved in anti-inflammatory,antioxidation,decreasing Phospho-IRS1 expression,and increasing Glut4 expression.