UPLC法同时测定香椿芽中8种黄酮醇苷

Simultaneous determination of eight flavonol glycosides in Toona sinensis shoots by UPLC

目的建立UPLC法同时测定香椿Toona sinensis Roemer芽中芸香苷、杨梅苷、金丝桃苷、异槲皮苷、番石榴苷、紫云英苷、槲皮苷、阿福豆苷的含有量。方法香椿芽60%甲醇提取液的分析采用Waters XBridge Shield RP18色谱柱(2.1 mm×150 mm,1.7μm);以乙腈-水为流动相,梯度洗脱;体积流量0.35 m L/min;检测波长350 nm;柱温35℃。结果 8种黄酮醇苷在各自范围内线性关系良好(r≥0.999 2),平均加样回收率98.3%~103.5%,RSD0.46%~3.36%。结论该方法准确稳定,重复性好,可用于香椿芽的质量控制。

AIM To establish a UPLC method for the simultaneous content determination of glycosides,rutinoside,myricitrin,hyperoside,isoquercitrin,guaijaverin,astragalin,quercitrin and afzelin in the shoots of Toona sinensis Roemer. METHODS The analysis of 60% methanol extract of T. sinensis shoots was performed on a35 ℃ thermostatic Waters XBridge Shield RP18 column（ 2. 1 mm × 150 mm,1. 7 μm）,with the mobile phase comprising of acetonitrile-water flowing at 0. 35 m L/min in a gradient elution manner,and the detection wavelength was set at 350 nm. RESULTS Eight flavonol glycosides showed good linear relationships within their own ranges（ r ≥ 0. 999 2）,whose average recoveries were 98. 3%-103. 5% with the RSDs of 0. 46%-3. 36%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of T. sinensis shoots.