不同产地15个品种淫羊藿的质量评价

Quality evaluation of fifteen varieties of Epimedii Folium from different growing areas

目的评价不同产地15个品种淫羊藿Epimedii Folium的质量。方法采用UPLC-MS/MS法,测定淫羊藿苷、淫羊藿次苷Ⅰ、淫羊藿次苷Ⅱ、朝藿定A、朝藿定B、朝藿定C、箭藿苷A、箭藿苷B、鼠李糖基淫羊藿次苷Ⅱ、宝藿苷Ⅱ的含有量。淫羊藿40%乙醇提取液的分析采用Phenomenex C18色谱柱(50 mm×2.1 mm,3μm);以0.1%甲酸-乙腈为流动相,梯度洗脱;体积流量0.2 m L/min;柱温40℃。再进行主成分分析。结果 10种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率97.6%~104.5%,RSD 1.37%~2.93%。46批样品中朝藿定C含有量最高,朝藿定A次之,淫羊藿次苷Ⅰ最低。小花、大花品种中各成分含有量有明显差异。结论该方法灵敏快速,可为淫羊藿开发利用提供依据。

AIM To evaluate the quality of fifteen varieties of Epimedii Folium from different growing areas.METHODS UPLC-MS/MS was adopted in the content determination of icariin,icarisideⅠ,icarisideⅡ,epimedin A,epimedin B,epimedin C,sagittatoside A,sagittatoside B,2″-O-rhamnosylicariside Ⅱ and baohuoside Ⅱ.The analysis of 40% ethanol extract of Epimedii Folium was performed on a 40 ℃ thermostatic Welch C18column（ 50 mm × 2. 1 mm,3 μm）,with the mobile phase comprising of 0. 1% formic acid-acetonitrile flowing at0. 2 m L/min in a gradient elution manner. Then principal component analysis was made. RESULTS Ten constituents showed good linear relationships within their own ranges（ r 0. 999 0）,whose average recoveries were97. 6%-104. 5% with the RSDs of 1. 37%-2. 93%. In 46 batches of samples,epimedin C content was the highest,followed by epimedin A content,and icarisideⅠcontent was the lowest. The contents of various constituents showed obvious differences between small-flowered taxa and big-flowered taxa. CONCLUSION This sensitive and rapid method can provide a reference for the development and utilization of Epimedii Folium.