Mcl-1与高尔基体磷酸化蛋白在结肠癌组织中的表达及与其临床病理特征的关系

Expression of Myeloid Leukemia-1 (Mcl-1) and Golgi Phosphor Protein (Golph3) in Colon Carcinoma and Its Relationship with Clinical Pathological Features

目的探究髓细胞白血病-1(Mcl-1)与高尔基体磷酸化蛋白(Golph3)在结肠癌组织中的表达水平及与其临床病理特征的关系。方法应用免疫组化方法,检测60例结肠癌组织中Mcl-1、Golph3表达水平,并与其癌旁正常组织中的表达水平进行比较。结果 Golph3、Mcl-1在正常组织及结肠癌组织中均有表达,然而在结肠癌组织中的阳性表达率显著高于癌旁正常组织(P<0.05);Mcl-1表达水平与患者性别、年龄、肿瘤分化程度、淋巴结转移无明显相关性(P>0.05),与肿瘤浸润深度、临床分期密切相关,浸润程度大,临床分期Ⅲ、Ⅳ期结肠癌组织中Mcl-1阳性表达率显著较高(P<0.05);Golph3水平与浸润深度、淋巴结转移密切相关,浸润程度大,发生淋巴结转移患者组织中Golph3阳性表达率明显较高(P<0.05)。结论 Mcl-1、Golph3参与结肠癌的发生、发展过程中,与正常组织相比其表达量显著升高,且与肿瘤远端转移、淋巴结转移,临床分期等病理参数密切相关。

Objective To investigate the expression of myeloid leukemia-1（ Mcl-1） and Golgi phosphor protein（ Golph3） in colon carcinoma and its relationship with clinical pathological features. Methods The expression level of Mcl-1 and Golph3 in 60 cases of colon cancer tissues were detected using immunohistochemical method,and compared with adjacent normal tissues. Results Mcl-1 and Golph3 in normal tissues and colorectal cancer tissues were expressed,but in colon cancer tissues the positive expression was significantly higher than normal tissues（ P〈0. 05）; there was no significant difference between the expression level of Mcl-1 in patients and gender,age,tumor differentiation,lymph node metastasis（ P〉0. 05）. But the expression level of Mcl-1 in patients was closely related to the clinical stage,the depth of tumor invasion. The positive expression rate of Mcl-1 in colon cancer with clinical stage Ⅲ,Ⅳ and depth of tumor invasion was significantly higher（ P〈0. 05）. The expression level of Golph3 was related to infiltration depth and lymph node metastasis,while Golph3 has significantly higher expression in lymph node metastasis tissues and the large degree of invasion（ P〈0. 05）. Conclusion Mcl-1,Golph3 are involved in the process of development colon cancer,and the expression significantly increased compared with the normal tissue,which are related to distal metastasis tumor,lymph node metastasis,clinical stage,pathology the parameters.