免疫组化法观察日本血吸虫感染破坏小鼠脾脏淋巴滤泡结构

Observation on destroyed architecture of splenic lymphoid follicles in mice infected with Schistosoma japonicum by immunohistochemistry

目的采用免疫组化(IHC)方法观察日本血吸虫感染破坏小鼠脾脏淋巴滤泡结构的现象。方法日本血吸虫感染小鼠(20条尾蚴/鼠)8周后剖杀,取脾脏样本石蜡包埋、切片,先以苏木素-伊红(HE)染色观察小鼠脾脏淋巴滤泡整体结构,再以IHC法用抗B220、CD21及Ki67抗体标记淋巴滤泡内B细胞、滤泡树突状细胞(FDC)及生发中心细胞,观察滤泡内具体细胞分布。结果 HE染色显示,感染日本血吸虫小鼠脾脏淋巴滤泡结构模糊难辨,相较于正常小鼠滤泡数目及面积均显著减少;IHC染色发现,日本血吸虫感染小鼠脾脏T、B淋巴细胞分区及FDC网络结构和生发中心结构均消失。结论日本血吸虫感染可显著破坏小鼠脾脏淋巴滤泡结构。

Objective To observe the destroyed architecture of splenic lymphoid follicles in mice infected with Schistosoma japonicum by immunohistochemistry. Methods The mice infected with S. japonicum（20 cercariae/mouse）for 8 weeks were sacrificed,and the splenic samples were paraffin embedded and sliced. The sections were first stained by hematoxylin and eosin to observe the massive structure of splenic lymphoid follicles,and then B cells,follicular dendritic cells（FDC）and germinal center cells were labeled with anti-B220,anti-CD21 or anti-Ki67 antibodies respectively by immunohistochemistry to observe the distribution of the specific cells of lymphoid follicles. Results The results of HE staining showed that the structure of lym-phoid follicles in spleens of infected mice was blurred,the number and area of follicles were significantly reduced compared to those of the normal mice. The immunohistochemical staining showed that the splenic T/B lymphocyte segregation ,FDC network and germinal centers of the infected mice all disappeared. Conclusion The structure of splenic lymphoid follicles in the mice infected with S. japonicum is obviously damaged.