海南三亚豇豆枯萎病病原菌鉴定及室内药剂筛选

Pathogenic Identification and Indoor Screening of Cowpea Fusarium Wilt in Sanya,Hainan Province

通过形态学观察、致病性测定和ITS序列分析对海南三亚地区采集的豇豆枯萎病病原菌进行鉴定,将豇豆枯萎病病原鉴定为尖孢镰刀菌(Fusarium oxysporum)。进行10种常用药剂对病原菌的毒力测定试验,结果表明:乙蒜素、甲基硫菌灵、代森锰锌3种药剂对豇豆枯萎病具有较好抑制作用,其EC_(50)值分别为47.42、33.58、74.26μg/mL;EC_(90)值分别为196.69、325.26和581.06μg/mL;a值分别为2.074 4、1.299 6和1.434 4,表明豇豆枯萎病病原菌对该3种药剂较为敏感。

Isolatesstrains of cowpea fusarium wilt collected from cowpea in Sanya were identified by using the morphological description, pathogenicity testing in vivo and ITS sequence analysis. These isolates were identified to be Fusarium oxysporum. The toxicity of 10 fungicides against the isolateswas tested. The results showed that ethylicin, thiophanate methyl and mancozeb had better inhibiting effect on fusarium wilt with their EC50 values being 47.42, 33.58, 74.26 μg/mL, respectively, and their EC90 values being 196.69, 325.26, 581.06 μg/mL, respectively, as well as their a values being 2.074 4, 1.299 6 and 1.434 4, respectively. This indicated that the isolates were sensitive to these three fungicides.