Rab1A对NSCLC细胞迁移和JAK1磷酸化的影响

Effect of Rab1A on the migraton of non-small cell lung cancer cell lines

目的:观察Rab1A(Ras-related protein Rab-1A)对非小细胞肺癌(non-small cell lung cancer,NSCLC)细胞系迁移的影响及其分子机制。方法:应用siRNA干扰Rab1A基因表达;应用RTCA(real-time cell analysis)法分析干扰Rab1A基因表达对NSCLC细胞迁移速率的影响;应用免疫印迹观察干扰Rab1A基因表达对JAK1(Janus Kinase 1)磷酸化水平的影响。结果:与阴性对照组相比,Rab1A siRNA转染组NSCLC细胞系的Rab1A蛋白表达水平显著下调(P<0.01);干扰Rab1A基因表达抑制NSCLC细胞迁移(P<0.01);干扰Rab1A基因表达可下调NSCLC细胞迁移相关蛋白JAK1磷酸化水平(P<0.01)。结论:Rab1A通过调节JAK1磷酸化水平影响NSCLC细胞迁移。

Objective ：To investigate the effects of RablA （Ras -related protein Rab -1A） on the migraton of non - small cell lung cancer （NSCLC） cell hnes and its molecular mechanism. Methods ：The siRNA interference was used to knockdown Rabl A. The effects of knocking Rabl A siRNA on the migraton of NSCLC cell lines were investiga ted by RTCA （real- time cell analysis）. Western blot was used to analyze the effects of RablA on the phosphorylation level of JAK1 （P - JAK1 ）. Results：Expression level of RablA protein was significantly down - regulated in Rabl A siRNA groups compared to negative control group （P 〈 0.01 ）. Rabl A siRNA knockdown inhibited the migraton of NSCLC cell lines in RablA siRNA groups compared to negative control group （ P 〈 0.01 ）. Phosphorylation ltev el of JAK1 （P- JAK1 ） was significantly decreased by RablA knockdown in RablA siRNA groups compared to nega- tive control group （P 〈 0.01 ）. Conclusion： RablA promotes the migraton of NSCLC cell lines through regulating phosphorylation level of JAK1 （ P - JAK1 ）.