隐丹参酮对表皮葡萄球菌生物膜的抑制作用

Inhibitory effect of cryptotanshinone on biofilm of Staphylococcus epidermidis

目的探索隐丹参酮对表皮葡萄球菌(SE)生物膜不同成熟阶段的抑制效果。方法体外构建SE生物膜模型,确定其黏附、聚集、成熟阶段时间点;通过半定量黏附实验、XTT法和扫描电镜检测不同浓度隐丹参酮作用下SE不同成熟阶段中生物膜基质量、膜内菌代谢活性和微观形态结构的变化。结果 SE生物膜黏附、聚集和成熟时间点分别为6、24、48 h;对于黏附阶段生物膜,128μg/mL和32μg/mL的隐丹参酮均能明显减少其生物膜基质量和杀灭膜内菌,差异有统计学意义(P<0.05),且隐丹参酮抑制作用128μg/mL优于32μg/mL,差异有统计学意义(P<0.05),同时均能破坏其微观形态结构;对于聚集与成熟阶段生物膜,仅128μg/mL的隐丹参酮能明显减少其生物膜基质量和杀灭膜内菌,差异有统计学意义(P<0.05),同时能破坏其微观形态结构,而32μg/mL的隐丹参酮则无明显抑制效果(P>0.05)。结论隐丹参酮对SE生物膜不同成熟阶段均具有一定抑制效果,且存在一定的剂量效应。

Objective To investigate the inhibitory effect of cryptotanshinone on different maturation stages of Staphylococcus epidermidis （S.epidermidis） biofilm.Methods The biofilm model of S.epidermidis was constructed in vitro, the timing of adhesion, accumulating, and maturation was determined;matrix quantity, bacterial metabolism, microstructure of biofilm were detected with semi-quantitative adhesion test, XTT assay, and scanning electron microscope（SEM） respectively.Results The timing of adhesion, accumulating, and maturation of S.epidermidis biofilm were 6h, 24h,and 48h respectively;in adhesion period, cryptotanshinone at the concentration of 128μg/mL and 32μg/mL could both obviously reduce the matrix and kill bacteria inside biofilm, difference was statistically significant（P〈0.05）,inhibitory effect of 128μg/mL cryptotanshinone was better than 32μg/mL （P〈0.05）, the microstructure was destroyed by both concentrations.During accumulating and mature period, only cryptotanshinone at 128μg/mL could reduce the matrix of biofilm and kill bacteria inside biofilm （P〈0.05）, the microstructure was damaged by cryptotanshinone at concentration of 128μg/mL, while 32g/mL of cryptotanshinone had no obvious inhibitory effect（P〉0.05）.Conclusion Cryptotanshinone has a certain inhibitory effect on different stages of S.epidermidis biofilm, and there is a certain dose effect.