基于GC-MS检测探讨橘属杂果皮代中药陈皮入药的可能性

Using GC-MS Method to Analyze the Possibility of Other Citrus Peels Instead of Pericarpium Citri Reticulatae

目的为陈皮药材来源提供理论基础与探讨橘属杂果皮代中药陈皮入药的可能性。方法单因素考察橘属挥发油的提取工艺。比较陈皮与新鲜橘皮、砂糖橘皮、柚皮挥发油的提取率。GC-MS检测陈皮、新鲜橘皮、砂糖橘皮挥发油中的主要成分。结果砂糖橘皮的挥发油提取率略高于橘皮,而砂糖橘和橘皮远高于柚皮。GC-MS检测结果显示砂糖橘皮挥发油中D-柠檬烯含量高于橘皮,新鲜橘皮中D-柠檬烯含量高于陈皮。结论砂糖橘作为常见橘属杂果皮不仅有成为陈皮的药材来源的可能性,而且还可作为医药行业提取D-柠檬烯的重要来源。放置一年以上的陈皮挥发油中的D-柠檬烯含量小于新鲜阴干的橘皮,这可能与陈皮在长期贮存期间挥发油中D-柠檬烯逐渐减少有关。

Objective In order to provide theoretical basis for Pericarpium Citri Reticulatae herbs resources and analyze the possibility of other citrus peels instead of Pericarpium Citri Reticulatae. Methods According to single factor experimental design, the influence of three parameters on the extraction rate of orange peel and pomelo peel were investigated importantly, including extraction time, solid-liquid ratio and particle size.D-limonene, the main component of volatile oil, was qualitative and quantitative analyzed by GC-MS method.Results The volatile oil extraction rate of sugar orange peel is slightly higher than orange peel but much higher than pomelo peel. The qualitative and quantitative analysis of the four kinds of volatile oil can be realized by GCMS method, which provide the content of D-limonene of sugar orange peel is higher than orange peel. Conclusion Sugar orange peel as a kind of citrus peels can be the source of Pericarpium Citri Reticulatae,but also become an important medicine source of extracting D-limonene.the content of D-limonene of fresh orange peel is higher than dried tangerine peel placed more than a year,which may related to the gradual decrease of D-limonene during long-term storage.