UPLC法测定六神曲发酵过程中噻嗪双酮苷及噻嗪二酮含量

Determination of Xanthiside and Xanthiazone in the Fermentation Process of Massa Medicata Fermentata by UPLC

目的建立UPLC法测定六神曲发酵过程中噻嗪双酮苷及噻嗪二酮的含量测定方法,监测六神曲的发酵过程、控制六神曲的质量。方法采用UPLC法测定不同发酵天数六神曲样品中噻嗪双酮苷及噻嗪二酮的含量。结果六神曲发酵过程中噻嗪双酮苷含量降低,而噻嗪二酮的含量呈先升高后降低的趋势。噻嗪双酮苷和噻嗪二酮分别在1.40~140μg/ml(r=0.999 1)和1.19~119μg/ml(r=0.999 1)内与峰面积成良好线性关系,平均回收率分别为99.5%和101.6%。结论 UPLC法作为测定六神曲发酵过程中噻嗪双酮苷及噻嗪二酮含量的一种手段,方法简便准确、重现性和稳定性较好,适于作为控制六神曲质量的方法,为六神曲的质量评价和控制提供了科学依据。

Objective To establish a method for determining the contents of Xanthiside and Xanthiazone in the fermentation process to control the quality of Massa Medicata Fermentata（ MMF）by ultra performanceliquid chromatography（ UPLC）. Methods Contents of the Xanthiside and Xanthiazone was measured by UPLC, which were in the fermentation process of MMF for seven days. Results It was showed that the content of xanthiside was reduced, but the content of xanthiazone was first increased and then decreased. The linear ranges were 1.40 to 140 μg/ml（ r = 0.999 1）, 1.19 to 119 μg/ml（ r = 0.999 1）, respectively. The average recovery were 99.5 %（ RSD = 1.6） for xanthiside and 101.6 %（ RSD = 1.9）（ n = 6） for xanthiazone, respectively. Conclusion The UPLC method was not only simple with good accuracy and reproductivity, but also could be applied for quality evaluation and control for Massa Medicata Fermentata.