血管生成拟态及VE-cadherin在肾透明细胞癌患者中的检测及其临床意义

The clinical significance of vasculogenic mimicry and expression of VE-cadherin in patients with clear cell renal cell carcinoma

目的探讨肾透明细胞癌(RCC)中是否存在血管生成拟态(VM)以及是否表达血管上皮钙粘附素(VE-cadherin),并进一步阐述VM与VE-cadherin的临床意义及二者与患者临床预后之间的关系。方法收集44例临床及随访资料完整的RCC患者的病例,进行过碘酸雪夫氏染色(PAS)与CD31双重染色以及VE-cadherin免疫组织化学染色法检测RCC组织中是否存在VM以及VE-cadherin的表达。结果 79.6%(35/44)的RCC患者存在VM,且VM与病理分级、临床分期显著相关(P<0.05),与患者年龄、性别、肾包膜浸润、转移及预后无关。RCC中VE-cadherin阳性率为65.9%(29/44),其表达与年龄、性别、临床分期、肾包膜浸润、预后无关,但与病理分级显著相关(P<0.05)。结论 RCC中存在VM以及VE-cadherin的表达,初步研究认为RCC的恶性度越高VM形成能力越强,VE-cadherin的表达也越高,VM与VE-cadherin之间存在正相关关系,推测他们与肾癌的恶性生物学行为有关。

Objectives To explore whether vasculogenic mimicry （VM） and vascular epithelium cadherin （VE-cadherin） exist in renal clear cell carcinoma （RCC）, and to explain the clinical significance of VM and VE-cadherin and their relationship with the prognosis of RCC. Methods A total of 44 RCC tissue specimens with complete clinical and prognostic data were ret- rospectively reviewed. The presence of VM in RCC was examined by periodic acid-schiff （PAS） staining with CD31 double stai- ning technique,and the expression of VE-cadherin was detected by immunohistochemical staining. Results VM was detected in 79.6 % （35/44） of RCC specimens. VM was significantly correlated to pathological grade and clinical staging （P〈0.05）, but not to age,sex,renal capsule invasion, metastasis and prognosis. The positive rate of VE-cadherin was 65.9% （29/44）. Its ex- pression was not related to age, sex, clinical stage, renal capsule infiltration and prognosis, but significantly to pathological grade （P〈0. 05）. Conclusions VM and VE-cadherin exist in RCC. The more invasive RCCs exhibit more VM and VE-cadherin expressions than the less vicious RCCs. VM is positively related to the expression of VE-cadherin, and they may contribute to the invasiveness of RCC.