响应面优化微波辅助提取海鲜菇废菌棒中多糖工艺

Study on the Microwave-assisted Extraction of Polysaccharides from Hypsizygus marmoreus Waste Substrate by Response Surface Methodology

采用微波辅助提取工艺从海鲜菇废菌棒中提取多糖,通过控制提取时间、微波功率以及液料比3个提取条件来优化提取工艺。在单因素试验基础上,结合响应面法,得出优化后的提取参数:提取时间10 min,微波功率420 W,液料比31∶1(mL/g)。在此条件下海鲜菇废菌棒多糖的提取效果最佳,提取率为2.01%。

The polysaccharides were extracted from the Hypsizygus marmoreus waste substrate by microwaveassisted extraction,and the extraction process was optimized by controlling the extraction time,microwave power and liquid-to-solid ratio. On the basis of single factor test and obtained by the response surface method,we got obtained by the response surface method：the extraction time was 10 min,the microwave power was420 W,and ratio of liquid to material was 31∶1（mL/g）. Under these conditions,the extraction efficiency of polysaccharide from waste substrate was the best,and the extraction rate was 2.01 %.