一种基于1,8-双取代芘的荧光探针对Cu^(2+)和焦磷酸根的接力识别

A Novel 1,8-Disubstituted Pyrene-Based Fluorescent Probe for Subsequent Detection of Cu^(2+) and Pyrophosphate

以1,8-二乙炔基芘和8-氨基喹啉为原料,通过click反应合成了一种双取代芘荧光探针L,表征了其结构.探针L可以在THF/H_2O(V∶V=1∶1,HEPES,1×10^(-2) mol/L,p H=7.4)中快速识别Cu^(2+),其他离子没有干扰.Job’s实验和高分辨质谱证明L与Cu^(2+)的结合计量比为1∶1,密度泛函理论计算表明L与Cu^(2+)形成了多元配合物.此外,L-Cu^(2+)对PPi具有良好的选择性和灵敏度,且响应快速,其检测限可达到6.32μmol·L^(-1),有潜在的应用价值.

A 1,8-disubstituted pyrene-based fluorescent probe L was designed and synthesized using 1,8-diethynylpyrene and 8-aminoquinoline as raw materials by ＂click＂ reaction and the structure of L was characterized. The probe L exhibited good selectivity and sensitivity to Cu^（2＋） with fast response and good anti-interference ability in the THF/H_2O（V∶V=1∶1, HEPES 1×10^（-2） mol/L, p H=7.4）. Job＇s plot and high resolution mass spectrometry showed that stoichiometric ratio of L to Cu^（2＋） was 1∶1, and the density functional theory calculations indicated that L with Cu^（2＋） formed a multicomponent complex. In addition, the L-Cu^（2＋） showed highly selective sequential recognition of PPi with good sensitivity and fast response. The detection limit of L-Cu^（2＋） to PPi could reach 6.32 μmol·L^（-1）, which illustrated L-Cu^（2＋） had the potential application value.