B7-H3 3＇-UTR SNP与胃癌发生相关性研究

Polymorphisms Within microRNA Binding-Sites in B7-H3 and Gastric Cancer

目的 探讨协同刺激分子B7-H3基因3＇-UTR SNP位点与胃癌发生相关关系.方法 采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）测定了183例胃癌患者和348名健康志愿者外周血中SNP位点rs1044581的基因型分布.结果χ2检验统计分析结果表明,B7-H3 SNP位点rs1044581与胃癌的发生潜在性相关（P=0.086）;rs1044581与胃癌患者肿瘤大小近似相关（P=0.073）.结论 B7-H3基因3＇-UTR SNP位点rs1044581与胃癌患者危险性有一定相关关系,未来有可能作为癌症诊断、治疗的一个新指标.

Objective To explore the association of the SNPs in the 3＇-UTR of B7-H3 gene with the risk of gastric cancer. Methods Genotype distribution of SNP rs1044581 was determined in 183 gastric cancer patients and 348 healthy volunteers by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Results Statistical analysis results showed that the SNP rs1044581 has a potential relation with the risk of gastric cancer （P=0.086）, and rs1044581 was potentially relevant to the tumor size of gastric cancer patients （P=0.073）. Conclusion The polymorphisms in the 3＇-UTR of B7-H3 have a certain relationship with gastric carcinogenesis.