摘要
为研究麦冬类植物材料的遗传多样性,利用非变性聚丙烯酰胺凝胶电泳技术,用8对EST-SSR引物在32份麦冬类植物材料进行扩增检测,并对样本进行聚类分析。结果表明:8对引物的PIC(Polymorphic information content)位于0~0.869,平均值0.567,引物1扩增出最多的等位基因数,占总值的20.41%;遗传距离矩阵研究发现,个体1和个体23间的遗传距离最大为2.48,说明二者遗传分化程度最大,个体18和个体23地理来源虽然不同,但二者间遗传距离最小,表明二者间有一定程度的基因交流现象;同时,居群c(野生杭麦冬)内的平均遗传距离最小为0.03,居群d(阔叶山麦冬)内的平均遗传距离最大为0.93。在聚类分析中,32份材料被分为2大类,在主坐标分析中,32份材料被分为3大类,2种分析结果具有相似性。研究表明EST-SSR标记可以很好地对麦冬类植物材料进行划分。
A total of 32 resources of Liriopogons which came from 9 different geographical origins were collected,the genetic diversity among them were studied by using 8 EST-SSR markers through SDS-PAGE. The results showed that： The polymorphism information content of 8 EST-SSR primers ranged from 0 - 0. 869 with average 0. 567. Primer 1 generated the most alleles,accounting for 20.41% of the total. The genetic distance internal populations was 0.03 - 0.93,the population c （Ophiopogonis japonicus in Hangzhou） had the nearest genetic distance, the population d （Liriopogons platyphylla） had the farthest genetic distance. The individual 1 was farthest to 23 indicating they had the greatest genetic differentiation. The individual 18 was nearest to 23 although their geographical origins were different, showing they had gene exchange to some extent. The cluster analysis and principal coordinates analysis divided Ophiopogonis and Liriope into two and three groups respectively, they had a certain level of similarity. This study showed that the 8 EST-SSR markers could be applied in the classification of Liriopogons materials.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2017年第9期12-20,共9页
Journal of China Agricultural University
基金
基金项目:重大新药创制(2012ZX09304006)
