三七总皂苷对大鼠局灶性脑缺血再灌注后IL-10及TNF-α表达的影响

Effects of panax notoginseng saponins on expression of IL-10 and TNF-α in rats with focal cerebral ischemiareperfusion

目的观察三七总皂苷(PNS)对局灶性脑缺血再灌注后大鼠脑组织白细胞介素-10(IL-10)及肿瘤坏死因子(TNF-α)含量变化的影响,探讨PNS对脑缺血再灌注损伤的保护机制。方法将30只成年雄性SD大鼠随机分为假手术组、缺血再灌注组和PNS组,每组10只。缺血再灌注组采用线栓法制作大鼠大脑中动脉栓塞模型。各组分别于连续灌胃4周,观察3 d后处死取出脑脑织,采用ELISA法、免疫组织化学法和半定量RT-PCR法检测各组缺血区脑组织IL-10和TNF-α表达的变化,并进行大鼠行为能力测试评分。结果与假手术组和缺血再灌注组相比,PNS组术后脑组织中IL-10的表达水平明显增高,TNF-α的表达水平明显下调,PNS组行为能力较缺血再灌注组明显提高。结论三七总皂苷对脑缺血再灌注大鼠的保护作用,其作用机制可能与上调IL-10、降低TNF-α的表达有关。

Objective To investigate the effects of panax notoginseng saponins（PNS） on the expression of IL-10 and TNF-α in the brain after focal cerebral ischemia-reperfusion injury in rats,and the mechanism was explored. Methods 30 male adult Sprague-Dawley rats were randomly divided into sham-operation group,isehemia-reperfusion gruup and PNS treatment group, 10 rats in each group. The middle cerebral artery occlusion（MCAO） was established with ischemia-reperfusion group. The rats received continuous gastric lavage for 4 weeks,who were observed for 3 days and then killed to the detect the expression of IL-10 and TNF-α in ischemic region by ELISA,immunohistochemistry and RT-PCR. The behavioral ability score in rats were tested. Results Compared with the sham-operation group and ischemia-reperfusion group,the expression of IL-10 was increased,while the expression of TNF-α was decreased significantly in PNS treatment group, behavior score of PNS treatment group increased significantly than that of ischemia-reperfusion group. Conclusion The panax notoginseng saponlns can reduce the neurons injury after focal cerebral isehemia-reperfusion injury in rats partly by regulating the expression of IL-10 and TNF-α in the brain.