红景天苷对H_2O_2诱导的人晶状体上皮细胞氧化应激损伤的保护作用

The protection of salidroside on oxidative stress induced in human lens epithelium cells

目的:探讨不同浓度红景天苷对H_2O_2诱导的人晶状体上皮细胞(human lens epithelium cells,HLEC)氧化损伤的影响。方法:HLEC传代培养,分为4组:对照组:以正常培养液培养;氧化损伤组:100μmol/L的H_2O_2作用12h;红景天苷低浓度组:10μmol/L红景天苷预处理24h后,加入H_2O_2作用12h;红景天苷高浓度组:100μmol/L红景天苷预处理24h后,加入H_2O_2作用12h。应用MTT法观察红景天苷对HLEC细胞活力的影响;倒置显微镜下观察并记录各组细胞形态变化;DCFH-DA荧光探针检测胞内ROS含量的变化;分光光度计检测细胞内超氧化物歧化酶(superoxid dismutase,SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)及丙二醛(malondialdehyde,MDA)的含量。结果:红景天苷能明显抑制H_2O_2诱导的HLEC细胞活力下降,抑制细胞内ROS生成,引起HLEC内SOD、GSH-P水平的升高及MDA水平的下降。结论:红景天苷通过降低细胞内MDA产生,提高细胞内SOD、GSH-Px含量抑制H_2O_2诱导的HLEC损伤,提示红景天苷可能在HLEC损伤的治疗中具有保护作用。

AIM： To explore the effect of different concentrations of salidroside on H2O2 induced oxidative stress damage in human lens epithelium cells（HLEC）.METHODS： HLEC were cultured and divided into negative control group： cultured in normal cultivation; oxidative damage group： treated with 100μmol/L H2O2 for 12h; Salidroside low concentration group： 10μmol/L salidroside treated for 24h and H2O2 treated for 12h; Salidroside high concentration group： 100μmol/L salidroside treated for 24h and H2O2 treated for 12h. MTT method was applied to observe the effect of salidroside on HLEC survival rate. Morphological change of each group were observed and recorded under inverted microscope. DCFH-DA fluorescent probe was applied to detect intracellular ROS changes; content of malondialdehyde（MDA）, superoxide dismutase（SOD）and glutathione peroxidase（GSH-Px）in supernatants were detected by pectrophotometer.RESULTS： Salidroside obviously inhibited H2O2-induced HLEC vitality decline, inhibited ROS generation in cells, causing SOD, GSH-Px levels increased and MDA levels decreased.CONCLUSION： Salidroside inhibited H2O2 induced HLEC injury by decreasing the intracellular MDA content levels and increasing SOD, GSH-Px content levels, which conclude that salidroside may have a certain role in the treatment of HLEC damage.