白兰氏鸡精减轻抑郁病症作用的研究

The Effect of Brand's Essence of Chicken on Alleviating the Depression

抑郁症是一类以情绪低落、乐趣丧失为核心症状的疾病。目前,临床研究发现,抑郁症往往伴随着昼夜节律的紊乱,但对此类临床表现的产生机制缺乏系统深入的研究。该文建立了慢性温和不可预见性应激(chronic mild unpredictable stimulation,CUMS)抑郁症大鼠模型。在诱导抑郁症模型大鼠过程中通过外源补充白兰氏鸡精(Brand’s essence of chicken,BEC),应用ELISA、荧光定量PCR等方法从生理水平、基因转录水平研究BEC对生物钟系统的影响以及是否具有减轻抑郁症症状的作用。研究结果表明,BEC能有效调控和稳定生物钟母钟[下丘脑视交叉上核(suprachiasmatic nucleus,SCN)]及子钟(肝脏、脂肪组织)中生物钟基因的表达时相,减缓由CUMS引起的探索行为活动下降以及血清中相关激素的分泌紊乱,从而减轻抑郁症相关症状及有效预防抑郁症的发生。

Depression is a kind of disease with the core symptoms of low mood and lack of interest. Recent clinical studies have shown that circadian rhythm disorder was accompanied in the depression group in most cases, while the mechanism of circadian clock in the regulation of depression has not been systematically studied. In the present study, we used SD rats to establish the depression model via a method of chronic mild unpredictable stimulation（CUMS）. Moreover, the chicken active ingredient Brand＇s essence of chicken（BEC） was supplemented to analyze its impact on the biological clock system and further explored the potential effect on alleviating the occurrence of depression by ELISA and Real-time PCR. The results showed that BEC could effectively regulate and stabilize the expression phase of clock genes in both suprachiasmatic nucleus（SCN） and peripheral clock including liver and adipose tissue. In addition, BEC treatment alleviated the decrease of exploratory activity and abnormal hormones secretion in serum caused by depression, which further contributed to the prevention and amelioration of depression.