鱿鱼墨黑色素提取物对肿瘤细胞的抑制作用

Inhibitory Effects of the Melanin Extracts From Squid Ink on Cancer Cells

为探索鱿鱼墨黑色素提取物(SIME)对体外培养的肿瘤细胞的抑制效应,通过四甲基偶氮唑盐(MTT)法和划痕试验比较SIME对人结肠癌细胞HCT-116、宫颈癌细胞Hela、肝癌细胞Hep G2、乳腺癌细胞MCF-7和前列腺癌细胞PC-3增殖及细胞迁移能力的影响。结果表明,SIME对肿瘤细胞形态有不同程度的影响,对肿瘤细胞增殖有浓度依赖性抑制作用,并具有抑制肿瘤细胞非定向迁移的作用。SIME对HCT-116和PC-3细胞增殖的抑制作用较好,72 h时两者半数抑制浓度IC50分别为0.144mg·m L^(-1)和0.485 mg·m L^(-1)。由此可见,SIME具有较强的抗肿瘤活性,该研究结果为鱿鱼墨高值化利用提供了一定的理论依据。

In order to investigate the inhibitory effects of SIME（squid ink melanin extracts） on cancer cells,in vitro test is applies with the anti-proliferative activity of SIME in human colon carcinoma HCT-116 cells,cervical cancer Hela cells,hepatoma Hep G2 cells,breast cancer MCF-7 cells and prostate cancer PC-3 cells being assessed using the MTT（methylthiazolyl tetrazolium） assay. The effects of SIME on the cell migration were measured by the scratch assay.The results showed that the cells morphology was changed by the treatment of SIME. The proliferation rates of the cells were all negatively related to the SIME concentration. SIME had inhibitory effects on the non directional migration of cancer cells. SIME showed a better inhibitory effect against PC-3 and HCT-116 cells with IC50（concentration of inhibitor required for 50% inhibition） values of 0. 144 and 0. 485 mg·m L（-1）. The results suggested that SIME have strong anti-tumor activities,which provides a theoretical basis for the utilization of squid ink.