凤丹油体膜蛋白基因PoOLE17.5的克隆与表达特性分析

Cloning and Expression Characteristics Analysis of PoOLE17.5 in Paeonia ostii

油体是油料作物种子储存脂肪的细胞器,油体膜蛋白是构成油体的重要组分,对维持油体大小和稳定起着重要作用。本研究以油体膜蛋白为对象,克隆了凤丹的油体膜蛋白基因(OLE),其cDNA全长783 bp,开放阅读框长507 bp,共编码169个氨基酸,蛋白的分子量为17.48 k D,故将该基因命名为PoOLE17.5。生物信息学分析显示,该基因编码的蛋白包含2个亲水性区域和1个疏水性区域,并具有1个保守结构域"Oleosin domain"。而后的表达模式显示,PoOLE17.5在凤丹种子发育过程中的表达水平逐渐上升,在种子接近成熟时表达水平最高,而后略有下降。这一结果有利于今后通过基因工程手段调控凤丹油体发育。

Oil bodies are organelles that store fat in oil crop seeds. Oleosin is an important part of the oil body,which plays an important role in maintaining the size and stability of the oil body. In this study, we studied the oleosin protein. And the Paeonia ostii oleosin gene（OLE） was cloned. The full-length c DNA of this gene was 783 bp and and it encodes 169 amino acids. The protein had a molecular weight of 17.48 kD, so it was named PoOLE17.5.Bioinformatics analysis showed that the protein encoded by this gene contained 2 hydrophilic and 1 hydrophobic regions, and had a conserved domain called ＂Oleosin domain＂. The expression profile analysis showed that the expression level of PoOLE17.5 first increased gradually during seed development and reached the highest level at near-mature stage, but then decreased slightly. This result was helpful for controlling the development of the oil body by genetic method.