皮质醇对激素依赖型乳腺癌细胞MCF-7增殖迁移影响及机制

Effect and mechanism of cortisol on proliferation and migration of hormone-related breast cancer MCF-7 cells

目的:研究皮质醇对激素依赖型乳腺癌细胞MCF-7增殖、迁移的影响及其可能的作用机制。方法:不同浓度皮质醇处理激素依赖型人乳腺癌细胞株MCF-7。采用MTS法检测不同浓度皮质醇对细胞增殖的影响;细胞划痕试验检测不同浓度皮质醇对细胞迁移的影响;蛋白质印迹法检测细胞增殖相关蛋白磷酸化m TOR(p-m TOR)以及迁移相关蛋白MMP-9、MMP-2蛋白表达变化情况。结果:MCF-7细胞在皮质醇剂量为10 nmol/L时增殖显著(P<0.05)、迁移增加(P<0.01)。并且在皮质醇剂量10 nmol/L时,MCF-7细胞表达细胞增殖相关蛋白p-m TOR(P<0.05),及细胞迁移相关蛋白MMP-9(P<0.05)、MMP-2(P<0.01)明显升高,差异具有统计学意义。结论:小剂量(10 nmol/L)皮质醇可以导致激素依赖型乳腺癌细胞增殖、迁移增加,其机制可能是通过激活经典m TOR通路诱导细胞增殖及上调MMP-9、MMP-9蛋白表达实现。

Objective：To investigate the proliferation and migration effect of cortisol on hormone-related breast cancer MCF-7 cells.Methods：MCF-7 cells, originated from a hormone-related breast cancer woman, were treated with different concentration of cortisol.The effect of cortisol on cell proliferation was determined by MTS assay and the rate of cell survival was calculated. The effect of cortisol on cell migration was examined by wound-healing assay.Immunoblot analysis detected the protein expression of phospho m TOR（p-m TOR）, which is involved in cell proliferation,and MMP-9, MMP-2,which are related to cell migration. Results： Cortisol at concentration of 10 nmol/L significantly increased cell proliferation（P〈0.05）and cell migration（P〈0.01）compared with the vehicle group.Furthermore, cortisol at concentration of 10 nmol/L obviously increased the protein expression involved in cell proliferation（pm TOR,P〈0.05） and cell migration（MMP-9, P〈0.05; MMP-2,P〈0.01）. Conclusion： Cortisol at concentration of 10 nmol/L effectively induced cell proliferation and cell migration by activating m TOR pathway and upregulating MMP-9 and MMP-2 protein expression.