let-7f-5p在不明原因复发性流产绒毛组织中的表达及其功能研究

The mechanism study of enhancer RNA inducing inflammatory response in unexplained recurrent spontaneous abortion

目的 :筛选与不明原因复发性流产(unexplained recurrent spontaneous abortion,URSA)相关的微小RNA(miRNA),探讨其在URSA发生中的作用及其可能机制。方法:通过对URSA绒毛组织进行miRNAs测序,初步筛选差异表达的miRNAs;利用实时荧光定量PCR,验证筛选所得miRNAs表达水平;利用DAVID软件对靶基因进行Pathway分析;利用实时荧光定量PCR,检测p53通路中靶基因及其下游基因的表达水平。结果:let-7f-5p在URSA绒毛组织中表达水平显著上升;let-7f-5p的靶基因富集于p53通路,通路中靶基因MDM-X和其下游靶标促生长基因MDM2水平显著下降,促凋亡基因p53表达水平显著上升。结论:let-7f-5p可能调控p53信号通路中MDM-X/MDM2/p53基因,促进细胞凋亡,最终导致URSA发生发展。

Objective： To screen the differently expressed miRNAs study the expression andfunction of let-7f-5p in unexplained recurrent spontaneous abortion（URSA） and investigate the roles of miRNAs played in URSA. Methods： The miRNA-sequencing was applied to screen miRNAs that differently expressed in URSA placental villous; The q RT-PCR was used to validate the miRNA＇s expression of screened miRNAs; The DAVID was applied to analyze pathway; The q RT-PCR was conducted to measure the expression of target genes in p53 pathway. Results： The expression of let-7f-5p was markedly up-regulatedhighly expressed in URSA placental villous; The targets of let-7f-5p were abundant in p53 pathway, and the expression of MDM-X and MDM2 was significantly down-regulated, while the expression of p53 was significantly up-regulated. Conclusion： let-7f-5p might promote cell apoptosis in URSA by regulating the expression of MDM-X/MDM2/p53 in p53 pathway.