摘要
为建立畜禽肉中鸡源性成分的快速检测方法,以线粒体DNA细胞色素C氧化酶亚基Ⅰ(COⅠ)基因序列为靶点,比较羊、牛、猪、兔、鸽、鹌鹑、鸡、鸭、鹅9种动物COⅠ基因的差异位点,设计筛选鸡特异性引物,进行常规聚合酶链式反应(polymerase chain reaction,PCR)和荧光定量PCR检测。结果表明:所设计的引物仅对鸡肉DNA模板有扩增条带和典型扩增曲线,且循环阈(cycle threshold,Ct)值为21.78,对其他动物DNA模板无扩增。方法特异性较强,灵敏度较高,达pg级,可以用于畜禽肉与肉制品中鸡源性成分的快速、有效、准确检测。
In this study, a quick method to identify ingredients of chicken origin in livestock and poultry meat was developed by polymerase chain reaction (PCR) and fluorescence quantitative PCR using mitochondrial DNA cytochrome C coxidase subunit Ⅰ (CO Ⅰ) gene as target gene. The mitochondrial DNA CO Ⅰ genes from chicken, duck, goose, pig, cattle, sheep, rabbit, pigeon and quail revealed distinct loci. Based on this, a set of chicken-specific primers was designed. The results showed that the designed primers could only amplify template DNA from chicken with a cycle threshold (Ct) value of 21.78. The PCR assay had a high specificity and sensitivity (at pg level) and it could be used to detect ingredients of chicken origin in livestock and poultry meat quickly and accurately.
出处
《肉类研究》
北大核心
2017年第8期44-48,共5页
Meat Research
基金
江苏省农业三新工程项目(SXGC[2017]256)
国家农产品质量安全风险评估项目(GJFP2017015)
关键词
鸡源性成分
线粒体DNA
COⅠ基因
荧光定量PCR
chicken origin ingredients
mitochondrial DNA
cytochrome C coxidase subunit I (CO I) gene
fluorescence quantitative PCR