龟鹿益神颗粒对慢性疲劳大鼠骨骼肌中PGC-1α的影响

Guilu Yishen Granule Can Improve Content of PGC-1α in Skeletal Muscle of CFS Model Rats

目的:研究慢性疲劳综合征的基本病机,建立慢性疲劳大鼠模型,观察龟鹿益神颗粒对慢性疲劳大鼠行为学指标和骨骼肌中过氧化物酶体增殖物激活受体γ辅激活因子1α(PGC-1α)的影响。方法:雄性SPF级SD大鼠40只,随机分为正常组、正常对照组、模型组、苁蓉益肾颗粒组、龟鹿益神颗粒组,每组8只。除正常组、正常对照组不造模外,其他3组采用慢性束缚、夹尾激怒和力竭游泳方法构建慢性疲劳大鼠模型。正常组和模型组按10 ml/(kg·d)给予生理盐水灌胃,正常对照组和龟鹿益神颗粒组按1 250 mg/(kg·d)给予龟鹿益神颗粒混悬液灌胃,苁蓉益肾颗粒组按417 mg/(kg·d)给予苁蓉益肾颗粒混悬液灌胃。每天观察并记录大鼠的体质量、饮水量、进食量、粪便形态及鼠毛色泽等情况,用ELISA法检测大鼠骨骼肌中PGC-1α的含量。结果:造模前,各组大鼠体质量、力竭游泳时间、直立次数、跨格次数无明显差异。造模后,与正常组比较,模型组、龟鹿益神颗粒组、苁蓉益肾颗粒组大鼠体质量、力竭游泳时间、直立次数、跨格次数显著减少,差异均有统计学意义(P<0.05)。给药后,与正常组比较,模型组大鼠体质量、力竭游泳时间、直立次数、跨格次数、骨骼肌中PGC-1α含量显著减少,正常对照组大鼠骨骼肌中PGC-1α含量显著升高,差异均有统计学意义(P<0.05);与模型组比较,龟鹿益神颗粒组和苁蓉益肾颗粒组大鼠体质量、力竭游泳时间、跨格次数、直立次数、骨骼肌中PGC-1α含量显著升高,差异均有统计学意义(P<0.05);与苁蓉益肾颗粒组比较,龟鹿益神颗粒组大鼠力竭游泳时间、骨骼肌中PGC-1α含量显著升高,差异均有统计学意义(P<0.05)。结论:运用慢性复合因素复制慢性疲劳动物模型具有可行性。龟鹿益神颗粒可以改善慢性疲劳大鼠身心疲劳状态,提高慢性疲劳大鼠骨骼肌中PGC-1α含量。

Objective： To study the basic pathogenesis of chronic fatigue syndrome（CFS）, establish CFS models, and to observe effect of Guilu Yishen granule on behavioral indexes and peroxisome proliferator-activated receptor γ coactivator-1α（PGC-1α） in skeletal muscle of CFS rats. Methods： Divided 40 SPF male rats into the normal group, normal control group,model group, Congrong Yishen granules group and Guilu Yishen granule group, 8 cases in each group. Except the normal group and the normal control group, the other three groups were all given chronic immobilization, tail pinch and exhaustive swimming so as to establish CFS model rats. The normal group and the model group was given saline of 10 ml/（kg·d） by gavage, while the normal control group and Guilu Yishen granule group was given 1 250 mg/（kg·d） suspension of Guilu Yishen granule by gavage,and Congrong Yishen granules group was given 417 mg/（kg·d） suspension of Congrong Yishen granules by gavage. Recorded body mass, drinking amount, dietary amount, shape of stool and fur color of the rats every day, and detected PGC-1α content in skeletal muscle with enzyme-linked immunosorbent assay（ELISA） method. Results： There was no significant difference in the body mass, exhaustive swimming time, times of standing up and times squares crossed in the all groups before modeling. After modeling, compared with the normal group, body mass, exhaustive swimming time, times of standing up and times of squares crossed in the model group, Congrong Yishen granules group and Guilu Yishen granule group were decreased significantly, the differences being significant（P〈0.05）. After medication, compared with the normal group,body mass, exhaustive swimming time, times of standing up, times of squares crossed and PGC-1α content in skeletal muscle mitochondria of the model group were decreased significantly; PGC-1α content in skeletal muscle mitochondria of the normal control group were increased significantly, the differences being significant（P〈0.05）. Compared with the model group,body mass, exhaustive swimming time, times of squares crossed, times of standing up and PGC-1α content in skeletal muscle mitochondria of Congrong Yishen granules group and Guilu Yishen granule group were increased significantly, the differences being significant（P〈0.05）. Compared with Congrong Yishen granules group, exhaustive swimming time and PGC-1α content in skeletal muscle mitochondria of Guilu Yishen granule group were increased significantly, the differences being significant（P〈0.05）. Conclusion： It is feasible to create CFS models by copying chronic multiple factors. Guilu Yishen granule can improve fatigue of CFS model rats and content of PGC-1α in skeletal muscle.