白内障患者房水中淀粉样蛋白含量及其与年龄的相关性研究

Correlation between age and content of β-amyloid protein in aqueous humor of patients with cataract surgery at different ages

目的探讨不同年龄白内障患者房水中淀粉样蛋白(β-amyloid protein,Aβ)含量及其与年龄的相关性。设计实验研究。研究对象行手术治疗的白内障患者210人,平均年龄(72.33±10.35)岁。方法对所有受试者进行全身及眼科检查,在白内障手术开始前前房穿刺抽取房水,运用ELISA法检测样本中Aβ_(1-40)、Aβ1-42含量。将所有受试者按年龄分为A组(<60岁)、B组(60~69岁)、C组(60~79岁)、D组(≥80岁)。比较各组房水Aβ_(1-40)、Aβ1-42含量的差别及其与年龄的关系。主要指标房水Aβ_(1-40)和Aβ1-42含量。结果房水中Aβ_(1-40)蛋白含量在A、B、C、D四个组分别为(80.91±9.33)、(85.81±8.97)、(85.46±11.22)、(82.25±10.41)ng/ml(F=2.399,P=0.069);Aβ42蛋白含量分别为(41.98±7.26)n、(42.46±5.75)、(43.57±5.27)、(42.80±6.04)ng/ml(F=0.719,P=0.543)。Aβ_(1-40)/Aβ1-42在A、B、C、D四个组分别为(1.97±0.34)、(2.04±0.23)、(1.98±0.27)、(1.95±0.33)(F=0.680,P=0.565)。结论不同年龄白内障患者房水中Aβ_(1-40)、Aβ1-42含量以及Aβ_(1-40)/Aβ1-42比值随年龄增长无明显改变。患者房水中上述淀粉样蛋白含量发生明显变化,则提示可能存在与淀粉样蛋白代谢障碍相关疾病。

Objective To explore the correlation between age and β-amyloid protein（Aβ） content of aqueous humor in patients undergoing cataract surgery at different ages. Design Experimental study. Participants 210 patients with cataract treated by surgery,which mean age was 72.33 ±10.35 years old. Method All participants underwent systematic and ophthalmic examinations. When cataract surgery, the sample of aqueous humor was extracted with anterior chamber paracentesis. The contents of Aβ_（1-40）, Aβ1-42 were detected with ELISA method. All the subjects were divided by age： group A（ 〈60 years）, group B（60-69 years old）, group C（60-79 years）,group D（≥80 years）. The differences of contents of Aβ_（1-40）, Aβ1-42 in aqueous humor were compared among different age groups, the relationship between the contents of Aβ_（1-40）, Aβ1-42 in aqueous humor and age was investigated. Main Outcome Measures The contents of Aβ_（1-40） and Aβ1-42 in aqueous humor. Results The content of Aβ_（1-40） in aqueous humor in group A, B, C, D was 80.91 ±9.33, 85.81±8.97, 85.46±11.22, 82.25±10.41 ng/ml respectively（F=2.399, P=0.069）. The content of Aβ1-42 in aqueous humor in group A, B, C, D was 41.98±7.26, 42.46±5.75, 43.57±5.27, 42.80±6.04 ng/ml（F=0.719, P=0.543）. Aβ_（1-40）/Aβ1-42 ratio in group A, B, C, D was 1.97±0.34,2.04±0.23, 1.98±0.27, 1.95±0.33（F=0.680, P=0.565）. Conclusions There were no significant difference of Aβ_（1-40）, Aβ1-42 and Aβ_（1-40）/Aβ1-42 ratio in aqueous humor of patients with cataract at different ages. The contents of Aβ-amyloid protein in aqueous humor have no obvious change with age. If the Aβ-amyloid protein content in the aqueous humor changes markedly, there may be a disease associated with impaired amyloid metabolism.