摘要
为了更好的保护并促进优异葡萄种质资源的有效区分和合理利用,利用SSR标记技术对酿酒葡萄品种(系)材料中选取的15份特异性资源进行亲缘关系的分析、分类、种质识别。从44对SSR引物中筛选出15对用于供试葡萄种质的SSR扩增,共扩增出74条带,其中多态性条带71条,多态性百分率为95.9%。根据SSR扩增结果,分析表明15份葡萄材料遗传距离的变异范围为0~1.092 4。UPGMA聚类分析表明,在遗传相似系数0.63处,可将15份供试材料分为2个类群,准确地检测出基因型之间的遗传背景与遗传关系。通过多态性谱带的有序编码转换,构建了15个葡萄品种的分子身份证系统,结果表明,利用SSR标记进行酿酒葡萄种质资源的鉴定和保护是可行的。
In order to protect and promote the effective differentiation and rational utilization of grape germplasm resources,15 kinds of different grapevine germplasm materials were selected to research the differences of genetic diversity,genetic relationship and the germplasm identification.Using 15 pairs of SSR primers screened out from 44 for PCR amplification,74 bands were generated among 15 grape germplasms.Among them,71 bands were polymorphic.The percentage of polymorphic alleles was 95.9%.Varieties of cluster analysis showed that 15 varieties tested were divided into two groups in the genetic similarity coefficient of 0.63,provides certain reference basis for breeding research.After raw data processing and filtering,molecular identification of each variety were produced.The results showed that it was feasible to identify and protect the wine grape germplasm resources using SSR markers.
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2017年第9期42-50,共9页
Journal of Agricultural Science and Technology
基金
现代农业产业技术体系专项(CARS-30-YZ-4)
博士后基金项目(BSH-2015JJ-002)资助
关键词
葡萄
SSR标记
聚类分析
分子身份证
grape
SSR markers
cluster analysis
molecular identification
