Nogo-A蛋白在一氧化碳中毒大鼠海马组织中的动态表达

Dynamic Expression of Nogo-A in Hippocampus of Rats after Carbon Monoxide Poisoning

目的:观察一氧化碳中毒后大鼠海马组织中Nogo-A蛋白动态表达情况,探讨Nogo-A蛋白在一氧化碳中毒后神经系统损伤中的作用与影响。方法:随机数字法将雄性SD大鼠30只分为正常对照组(NC组)、CO中毒组(CO组)、CO中毒后24小时组(CO-24h)、CO中毒后48小时组(CO-48h)、CO中毒后7天组(CO-7d),每组各6只。CO气体腹腔注射染毒法建立一氧化碳中毒模型。建模后不同时间点利用免疫组织化学染色(IHC)、蛋白质免疫印迹技术检测(WB)Nogo-A蛋白在海马组织中的表达情况并分析其变化规律。结果:IHC结果表明NC、CO组、CO-24h组、CO-48h组、CO-7d组中Nogo-A蛋白平均光密度值分别为0.0928±0.0038、0.01172±0.0042、0.1452±0.0056、0.1271±0.0057、0.1088±0.0055;WB结果提示一氧化碳中毒后海马组织中Nogo-A蛋白的表达较NC组增高,在24h时表达达到高峰(P<0.05);24h后表达开始逐步下降,至染毒第7天时,海马组织中Nogo-A蛋白的表达明显下降,但仍高于NC组(P<0.05)。结论:在本研究中,大鼠海马组织中Nogo-A蛋白表达的增高与一氧化碳中毒相关;Nogo-A蛋白表达在24h时表达达到高峰;24h后表达开始逐步下降。

Objective： To study the dynamic expression of Nogo-A in hippocampus of rats after carbon monoxide poisoning, and to explore the effect and influence of Nogo-A in the damage to nervous system after carbon monoxide poisoning. Methods： Thirty male SD rats were randomly divided into NC group（n=6）, CO group（n=6）, CO-24 h group（n=6）, CO-48 h group（n=6）, CO-7d group（n=6）. The method of injection CO gas was used to establish the carbon monoxide poisoning model. Then immunohistochemical （IHC） and Western blot （WB） techniques were used to observe dynamic expression of Nogo-A in hippocampus of rats at several time intervals after carbon monoxide poisoning and to analyze its change law. Results： IHC results showed that the average optical density value of expression of Nogo-A in NC group, CO group, CO-24 h group, CO-48h group and CO-7d group were 0.0928± 0.0038, 0.01172± 0.0042, 0.1452± 0.0056, 0.1271 ± 0.0057, 0.1088 ± 0.0055. WB results showed that the expression of Nogo-A in hippocampus after carbon monoxide poisoning was significantly higher than that in NC group（P〈0.05）, and reached the highest level at 24 h, then had a gradual recovery after 24h. The expression of Nogo-A decreased obviously, but still higher than that of NC group by day 7 （P〈0.05）. Conclusions： In this study, the increase of expression of Nogo-A was associated with carbon monoxide poisoning. The expression of Nogo-A reached the highest level at 24h, then had a gradual recovery after 24 h.