不同来源肠炎沙门菌毒力基因特征分析

Analysis of the characteristics of Salmonella enteritidis virulence genes from different origins

目的研究石家庄地区不同来源的肠炎沙门菌毒力基因携带情况的差异及特点。方法收集石家庄地区早市生禽销售点、生禽屠宰场及食物中毒标本中分离出的肠炎沙门菌124株,对其中8种毒力基因(invA、sopE、agfA、spvR、hilA、stn、pefA、shdA)进行聚合酶链式反应(PCR)检测。结果肠炎沙门菌具有不同的毒力基因谱型,8种毒力基因在不同来源的肠炎沙门菌中均有检出,其中毒力基因invA、sopE、stn、hilA、spvR、pefA在食物中毒分离株中的携带率高,均在94%以上。食物中毒分离株与早市生禽销售点分离株的8种毒力基因携带率差异无统计学意义(P>0.05),而生禽屠宰场分离株与食物中毒和早市生禽销售点分离株之间差异有统计学意义(P<0.05)。结论石家庄地区早市生禽销售点检出的肠炎沙门菌中易致病的高风险株较多,应加强对生禽的卫生监督与防控。

Objective To investigate the differences and characteristics of virulence genes carried by Salmonella enteritidis from different sources in Shijiazhuang City. Methods One hundred and twenty-four strains of Salmonella enteritidis isolated from morning markets of raw and poultry stalls,slaughterhouses and food poisoning specimens in Shijiazhuang area were collected. Eight virulence genes（ inv A,sop E,agf A,spvR,hil A,stn,pef A,shd A） were detected by polymerase chain reaction（ PCR）. Results Salmonella enteritidis might have different virulence gene profiles. The above eight virulence genes were detected in different strains. The carrying rate of virulence genes inv A,sop E,stn,hil A,spvR and pef A in the food poisoning strains was higher than 94%. There was no difference in the carrying rate of 8 virulence gene between the morning raw poultry stalls isolates and the patient strains,but was different with the slaughterhouse strains.Conclusion There were more risks of food poisoning caused by Salmonella enteritidis from morning markets,and the hygiene supervision should be strengthened to prevent and control foodborne disease.