乳腺癌组织中人叉头框蛋白C2表达的临床意义及其与细胞增殖能力的关系

Clinical Significance of Expression of Forkhead Box Protein C2 in Human Breast Cancer Tissues and Its Relationship with Amplification Capacity of Cells

目的观察人叉头框蛋白C2(Forkhead box protein C2,FOXC2)在乳腺癌组织中的表达水平,并探究其临床意义和与细胞增殖能力的关系。方法反转录-聚合酶链反应(RT-PCR)检测36例临床收集的乳腺癌组织及癌旁组织中FOXC2的表达情况,免疫组化检测肿瘤组织中ER、PR、Her-2的表达。对患者FOXC2的表达与患者一般情况及乳腺癌TNM分期、分子分型行Spearman相关性分析。体外转染干扰质粒干扰乳腺癌细胞MCF-7的FOXC2表达并经RT-PCR验证,MTT检测细胞增殖能力的变化。kaplan-meier生存分析检测患者的预后与FOXC2表达的关系。结果乳腺癌组织FOXC2表达显著高于癌旁组织,差异有统计学意义(P<0.05),其表达与乳腺癌TNM分期及分子分型存在显著的相关性(r=0.343、r=0.528,P<0.05),与患者年龄及体质量无显著相关(r=0.179、r=-0.138,P>0.05)。siRNA干扰MCF-7的FOXC2表达后,细胞增殖能力在48h及72h时降低,差异有统计学意义(P<0.05)。Kaplan-meier生存分析曲线示高表达FOXC2的患者预后不良。结论 FOXC2在乳腺癌组织中高表达,与乳腺癌进展、恶性程度及患者预后存在显著相关性,并能促进细胞的增殖,可能在乳腺癌的发生发展中起着重要的促进作用。

Objective To observe the expression level of forkhead box protein C2 （FOXC2）in breast cancer tissues and investigate its clinical significance and relationship with amplification capacity of cells. Methods Reverse-transcription polymerase chain reaction（RT-PCR） was adopted to determine the expression of FOXC2 in 36 clinical cases of breast cancer tissue and pa- racancerous tissue. And immunohistochemistry was used to test the expression of ER, PR and Her-2 in tumor tissues. Spearman correlation analysis of the expression of FOXC2 and the general situation of patients and the TNM staging and molecular typing of breast cancer was performed. Interference plasmid in vitro transfection disturbed expression of FOXC2 in breast cancer MCF-7 cell and it was verified by RT-PCR. MTI＂ assay was applied to detect the change of amplification capacity of cells. Kaplan-Meier surviv- al analysis was used to check the relationship between prognosis and the expression of FOXC2. Results The expression of FOXC2 in breast cancer tissue was significantly higher than that in paracancerous tissue（ P 〈 0. 05）and it had significant correlation with TNM staging and molecular typing of breast cance（ r =0. 343 ,r =0. 528 ,P 〈0.05） while it had no remarkably correlation with age and weight（ r = 0. 179 ,r = -0. 138 ,P 〉 0.05 ）. After the expression of FOXC2 in MCF-7 disturbed by siRNA, amplification capacity decreased at 48h and 72h（P 〈 0. 05 ）. Kaplan-Meier survival analysis curve showed that patients with high expression of FOXC2 had poor prognosis. Conclusion High expression of FOXC2 in breast cancer tissues is significantly related to the development and malignancy of breast cancer and patient＇s prognosis. And it can promote the proliferation of cells, which plays an important role in the carcinogenesis and development of breast cancer.