GPx5在内蒙古绒山羊附睾中的表达

Expression of GPx5 in the epididymis of Inner Mongolia Cashmere goat

为研究GPx5在内蒙古绒山羊附睾中的表达模式,对初情期、体成熟期、老龄期3个生殖生理阶段绒山羊附睾及睾丸中的GPx5从mRNA和蛋白水平进行了检测。结果显示各生殖生理阶段绒山羊睾丸中均未见GPx5表达,附睾中均有GPx5表达。各生殖生理阶段附睾表达区域及表达量存在差异:初情期,GPx5 mRNA和蛋白主要表达于附睾头部;体成熟期,附睾各区域GPx5 mRNA和蛋白的表达量极显著高于其他阶段,附睾起始部和附睾头部为主要表达区;老龄期,GPx5 mRNA和蛋白主要表达于附睾头部,但较体成熟期明显减少。免疫组化定位显示GPx5蛋白主要分布于附睾上皮细胞,而且各年龄阶段绒山羊附睾腔内精子上都有明显的GPx5阳性信号。研究表明绒山羊附睾GPx5的表达区域及表达量变化与动物生殖生理阶段直接相关。

In order to study the expression pattern of GPx5 in the epididymis of Inner Mongolia Cashmere goat,GPx5 mRNA and protein in epididymis and testis were detected at three reproductive physiology stages; Puberty stage,body mature stage and aged stage. The results showed that GPx5 was expressed in the epididymis of Cashmere goat in all reproductive physiology stages, but not in testis. However,there were differences in the expression region and quantity of epididymis in the three stages. At the puberty stage,GPx5 mRNA and protein were mainly expressed in epididymal caput. At the body mature stage, the expression of GPx5 mRNA and protein in each epididymal region was significantly higher than that in other stages. Initial segment and epididymal caput were the main expression regions. At the aged stage,GPx5 mRNA and protein were mainly expressed in epididymal caput and a significant expression level reduction was detected compared to that in body mature stage. Immunohistochemistry results showed that GPx5 protein mainly existed in epididymal epithelial cell and obvious GPx5 positive signals in epididymal sperm of Cashmere goat at different ages. These results indicated that the expression region and quantity of GPx5 in epididymis were directly related to the reproductive physiology stage of Cashmere goat.