红曲对去卵巢大鼠骨质疏松模型血清ALP,TRAP及骨组织TNF-α,RANK表达的影响

Effect of Red Yeast Rice on the Expression of Serum ALP and TRAP,TNF-α and RANK of Bone Tissue in Ovariectomized Osteoporosis Rats

目的:明确红曲对去卵巢大鼠骨质疏松模型血清ALP,TRAP及骨组织TNF-α,RANK表达的影响。方法:取12周龄Wistar雌性大鼠40只,随机分为结合雌激素组、红曲组、模型组、假手术组,每组10只。前三组行完整双侧卵巢摘除术,假手术组仅打开腹腔,不切除卵巢。灌胃12周后,处死各组动物,采用多光谱成像系统检测骨密度,用ELISA法测定各组大鼠血清中ALP及TRAP,用Image Pro Plus对免疫组化染色图像结果进行定量分析。结果:红曲组TNF-α免疫组化表达水平低于模型组(P<0.05),红曲组RANK免疫组化表达水平低于模型组(P<0.05)。结论:红曲可以提高血清ALP表达水平,降低血清TRAP表达水平,能够通过降低TNF-α水平及RANK水平来提高去卵巢大鼠骨密度,其机理与雌激素类似,可能与成骨细胞及破骨细胞活跃程度有关。

Objective：To investigate the effect of red yeast rice on the expression of serum ALP and TRAP,TNF-αand RANK of bone tissue in ovariectomized osteoporosis rats.Methods：Forty female Wistar rats of 12 weeks old were randomly divided into the combination estrogen group,the red yeast rice group,the model group and the sham-operation group,with 10 rats in each group.The first three groups were treated with completely bilateral ovariectomy,while the sham-operation group were only treated to open the abdominal cavity without removing the ovaries.After 12 weeks of gavage administration,the animals of each group were sacrificed.The bone mineral density was measured by multi-spectral imaging system,the serum ALP and TRAP of rats in each group were measured by ELISA method,and the results of immunohistochemically staining image were quantitatively analyzed by Image Pro Plus.Results：The immunohistochemically expression level of TNF-αand RANK in the red yeast rice group were lower than that in the model group（P〈0.05）.Conclusion：Red yeast rice can improve the expression of serum ALP and reduce the expression of serum TRAP.It can improve the bone density of ovariectomized rats by reducing TNF-αand RANK levels.The mechanism is similar to that of estrogen,which may be related to the osteoblast and osteoclast activity.