辣椒疫霉效应分子RxLR121504功能特性的研究

Functional analysis of an effector Rx LR121504 in Phytophthora capsici

辣椒疫霉菌Phytophthora capsici引起的辣椒疫病是世界性蔬菜病害,该病害严重发生常给辣椒生产造成严重损失。植物病原卵菌侵染寄主植物过程中常分泌大量的效应分子来促进自身的侵染与定殖,其中Rx LR效应分子在病原卵菌侵染寄主及与寄主植物互作过程发挥着重要的作用。辣椒疫霉菌是一种重要的植物病原卵菌,本研究以辣椒疫霉菌标准菌株LT1534为材料,克隆鉴定了辣椒疫霉的一个效应分子,编号为Rx LR121504,然后将其构建至PBIN‐GFP2植物表达载体,利用农杆菌介导的瞬时表达技术、Western blot和亚细胞定位观察技术,较深入地开展了Rx LR121504功能特性的研究。结果表明,Rx LR121504能有效引起本氏烟寄主的过敏性坏死反应(HR),并对激发子INF1诱导的细胞坏死反应具明显的抑制效果,因此Rx LR121504可能参与了辣椒疫霉菌抑制寄主的免疫抗菌过程。但Rx LR121504对寄主植物的分子靶标尚未鉴定明确,该效应分子对寄主植物的分子机制有待进一步深入的研究。

Phytophthora blight of capsicum caused by P capsici is a worldwide disease in vegetable production, and its serious occurrence causes great economic losses. P. capsici secretes plenty of effectors to promote the infection and colonization while the host plant is infected. RxLR effectors play an important role in infecting the host and interaction between P. capsici and plants. In this research, P. capsici LT1534 is used as a model to clone an effector named RxLR121504 and the effector is fused to plant expression vector PBIN-GFP2. The function Agrobacterium-mediated transient expression, Western of RxLR121504 was investigated by using the techniques of blot and subcellular localization. The results show that RxLR121504 could efficiently induce hypersensitive response （HR） in Nicotiana benthamiana, and suppress cell death induced by elicitor INF1 Thus, RxLR121504 may participate in the process of pathogen-host interaction. However, the molecular target of RxLR121504 to host plant remains to be identified and its molecular mechanism is required to further study out