大豆脯氨酸生物合成相关基因Glyma01g24530的功能预测及表达分析

Functional Prediction and Expression Analysis of Proline Synthetase Related Gene Glyma01g24530

干旱胁迫下的脯氨酸积累在许多植物中都存在,人们普遍认为脯氨酸含量的提高促进了植物对干旱胁迫的抵抗能力。拟南芥中研究发现,△1-吡咯啉-5-羧酸合成酶基因P5CS1是一种限速酶用来催化脯氨酸生物合成,对脯氨酸非常重要。本研究通过生物信息学及荧光定量PCR对大豆中的P5CS1同源基因Glyma01g24530进行了初步的功能分析和表达模式验证。结果发现:Glyma01g24530具有保守的N端乙酰谷氨酸合成酶激酶结构域和N端乙酰谷氨酸合成酶激酶结构域,系统进化树显示与各植物中P5CS家族成员相似性很高,启动子顺式作用元件序列分析表明该基因存在逆境胁迫、光反应等元件。表达模式分析显示Glyma01g24530可以被干旱、盐诱导表达,并在多数组织中表达。

Free proline can accumulate to high levels under drought-stress in many plants, and it is widely acknowledged that the increase of proline content could promote the plant resistant to drought stress. The study in Arabidopsis thaliana found that △1-pyrroline-5-carboxylate synthetase （P5CS） catalyzed the rate-limiting enzyme in the biosynthesis of proline and played an important role in the biosynthesis of proline. In this study, function and expression of P5CS1 homologous gene in soybean G/y- ma01924530 was analyzed through bioinformatics and real-time quantitative PCR. We found that GlymaOlg24530 had conser- vative N-acetylglutamate synthase, kinase-like domain and Aldehyde dehydrogenase domain structure. Phylogenetic tree showed that G/yma01g24530 had high similarity with PSCS1 family members in many plants. We also found that there were multiple cis-elements about abiotic stress responses, light responsiveness in its promoter sequence region through the statistics of promoter cis-elements. Expression pattern analysis showed G/yrna01g24530 could be induced by drought and salt, it could express in many tissues.