天山假狼毒根的化学成分研究

Chemical constituents from roots of Stelleropsis tianschanica

采用柱色谱方法对天山假狼毒Stelleropsis tianschanica根的95%乙醇提取物进行分离纯化,利用波谱技术对分得到的化合物进行结构鉴定。从天山假狼毒中分离得到了17个化合物,分别鉴定为5'-甲氧基落叶松脂醇(1)、松脂素(2)、双白瑞香素(3)、acutissimalignan B(4)、(+)-开环异落叶松树脂酚(5)、(+)-表松脂酚(6)、7-甲氧基西瑞香素(7)、thero-8S-7-methoxysyringylglycerol(8)、1-O-methyl-guaiacylglycerol(9)、2R-22'-阿魏酸酯-2,3-二羟基丙基酯(10)、vesiculosin(11)、4β,5βHguai-9,7(11)-dien-12,8-olide-1α,8α-diol(12)、(-)-nortrachelogenin(13)、4α,5βH-guai-9,7(11)-dien-12,8-olide-1α,8α-diol(14)、罗汉松脂素(15)、落叶松树脂素(16)和异落叶松树脂醇(17)。其中化合物1~13为首次从该属植物中分离得到。采用免疫荧光检测技术化合物3,7,10~14对孤儿核受体TR3的激活作用进行了考察,结果表明在50μmol·L^(-1)下,化合物10的TR3激活率高达76.38%,化合物3,7,11~14的能力较弱,均在50.0%左右。

The constituents from 95% ethanol extract of the roots of Stelleropsis tianschanica were purified by column chromatography techniques,leading to the isolation of 17 compounds. Their structures were elucidated by spectroscopic dataas 5 ＇-methoxy lariciresinol（ 1）,pinoresinol（ 2）,daphnoretin（ 3）,acutissimalignan B（ 4）,（ ＋）-secoisolariciresinol（ 5）,（ ＋）-epipinoresinol（ 6）,7-methyi-daphnoretin（ 7）,thero-8 S-7-methoxysyringylglycerol（ 8）,1-O-methyl-guaiacylglycerol（ 9）,2 R-22 ＇-ferulic acid ester-2,3-dihydroxypropyl ester（ 10）,vesiculosin（ 11）,4 β,5 βH-guai-9,7（ 11）-dien-12,8-olide-1 α,8 α-diol（ 12）,（-）-nortrachelogenin（ 13）,4α,5βH-guai-9,7（ 11）-dien-12,8-olide-1α,8α-diol（ 14）,matairesinol（ 15）,lariciresinol（ 16）and isolariciresinol（ 17）. Among them,compounds 1-13 wereobtained for the first time fromthe genus Stelleropsis. Compounds3,7,10-14 were tested for their activation of orphan nuclear receptor TR3 with the immunofluorescence technology in 50 μmol·L^（-1）. The results showed that compound 10 displayed moderate activity with the activity ratio of 76. 38 %,and the others were only about 50. 0 %.