SDF-1在梗阻性黄疸大鼠不同组织中的表达

Expression of SDF-1 in obstructive jaundice rat various tissues

目的动态观察梗阻性黄疸大鼠血浆、骨髓、肝脏、肺脏、肾脏中的表达变化,探讨其在梗阻性黄疸及其相关并发症中的意义。方法选择200 g左右雄性SD大鼠48只,分为假手术组(Sham组)、胆总管结扎组(CBDL组),分别于术后7、14、21 d,检测血清丙氨酸氨基转移酶(ALT)、谷草转氨酶(AST)、血清总胆红素(TB);观察两组大鼠一般情况;采用ELISA检测各时间点血浆及匀浆组织上清液中SDF-1的表达水平,并采用q PCR测定不同时间点各组织SDF-1 m RNA的表达水平。结果 CBDL组术后ALT、AST、TB迅速增高,与Sham组比较差异有统计学意义(均P<0.05)。CBDL大鼠血浆、肝组织、肺组织中SDF-1蛋白及m RNA表达量在不同时间点均明显高于Sham组,而肾组织中SDF-1的表达无明显差异,并且CBDL组大鼠骨髓SDF-1的表达在7、14、21 d明显低于Sham组。结论 SDF-1在梗阻性黄疸大鼠肝和肺组织中表达明显增高,在骨髓中表达降低,可能在促进骨髓相关干细胞动员及参与梗阻性黄疸肝肺病变中起到重要作用。

Objective To dynamically observe the SDF-1 level in plasma, bone marrow, liver, lung and kidney, and to investigate their significance in obstructive jaundice and its complications. Method 48 male SD rats weighing about 200g were randomly divided into Sham group and CBDL group. The serum ALT, AST and serum total bilirubin （TBIL） were detected at 7 d, 14 d and 21 d after operation. General condition of rats in the two groups were observed. ELISA was applied in detecting expression of SDF- 1 in plasma and supernate of tissue homogenate. And mRNA expression of SDF-1 at different time was detected by qPCR assay. Results ALT, AST,TB increased rapidly after CBDL operation, the difference was significant compared with Sham group （P 〈 0.05）. The SDF-1 expression of plasma, liver tissue, lung tissue in CBDL rats at different time points were significantly higher than in Sham group. No significant difference was found in renal tissue. SDF- 1 expression of bone marrow in 7 d, 14 d, 21 d was significantly lower in CBDL group than in Sham group. Conclusion Expression of SDF-1 in liver and lung tissues of obstructive jaundice rats significantly increased, and decreased in marrow bone. This change may promote related stem cells mobilization and contribute to the pathological changes of obstructive jaundice.