脑组织S100B蛋白及脑源性神经营养因子对胎鼠宫内脑损伤的预测价值

The Predictive Value of S100B Protein and BDNF in Brain Tissue for Fetal Rats Intrauterine Brain Injury

目的:寻找胎鼠宫内脑损伤的有效预测指标。方法:比较正常对照组和缺氧组胎鼠脑组织形态学变化、脑组织中S100B蛋白和脑源性神经营养因子(BDNF)的表达及含量。结果:(1)脑组织形态学结果显示:缺氧组胎鼠大脑皮质和海马区细胞排列紊乱,细胞数目减少,结构模糊或消失,组织间质疏松水肿。(2)免疫组织化学染色结果显示:S100B蛋白主要在海马区的齿状回部位表达,BDNF主要在海马区和皮质区表达。缺氧组S100B蛋白及BDNF阳性细胞平均灰度值较正常对照组明显增强(189.90±31.66 vs 84.66±12.90,P=0.000;139.04±15.83 vs 74.86±14.08,P=0.000)。(3)酶联免疫吸附试验检测结果显示:缺氧组胎鼠脑组织中S100B蛋白、BDNF含量均明显高于正常对照组(3.17±1.07 ng/ml vs 2.25±1.20 ng/ml,P=0.006;152.08±36.29 pg/ml vs 128.21±43.43 pg/ml,P=0.039)。结论:孕鼠缺氧后可导致胎鼠脑损伤,脑组织中S100B蛋白、BDNF的测定可作为预测胎鼠宫内脑损伤的指标。

Objective：Looking for effective predictors of fetal rats＇ brain injury. Methods：Observe the morpho- logic change of fetal rats＇ brain tissues,and the expression level of S100B protein and brain derived neurotrophic factor in brain tissues. Results.（1）After hypoxic cerebral, the cortex and hippocampus cells arranged disorder, the number of cells were reduced,the structure was fuzzy or disappeared, and the tissue stroma was edema. （2）lmmunohistochemical staining results suggested S100B protein is mainly expressed in the dentate gyrus of hippo- campus,BDNF is mainly expressed in the hippocampus and cortical regions. The expression of S100B protein and BDNF positive cells in hypoxia group was obviously increased compared with control group（189.90 ±31.66 vs 84.66 ± 12.90 ; 139.04± 15.83 vs 74.86 ±14. 08）, differences were statistically significant（ P = 0. 000）. （3）Enzyme-linked immunosorbent assay results showed that the content of S100B protein and BDNF in fetal rats＇ brain tissues in hypoxia group were significantly higher than in control group（3. 17 ±1.07 ng/ml vs 2.25 ±1.20 ng/ml; 152.08 ± 36.29 pg/ml vs 128.21 ± 43. 43 pg/ml）, differences were statistically significant （ P = 0. 006, P = 0. 039）. Conclusions： Fetal rats＇ brain injury occurred when pregnant rat experienced hypoxia. The detection of S100B and BDNF in brain tissues can be used to predict fetal rats＇ intrauterine brain injury,