新疆喀什地区母乳中乳酸菌的多样性分析

Biodiversity of lactic acid bacteria isolated from human breast milk of Kashi area in Xinjiang

【目的】对新疆喀什地区母乳中乳酸菌多样性进行分析。【方法】采用菌落培养、显微镜观察、Repetitive genomic fingerprinting(Rep-PCR)指纹图谱和16S r RNA基因序列分析相结合的方法研究母乳中乳酸菌的菌群分布。【结果】从11份母乳中共分离出乳酸菌193株,利用16S r RNA基因序列同源分析和系统发育树对代表菌株进行了分子鉴定,193株乳酸菌隶属于4个属,分别为Lactobacillus(22株)、Streptococcus(42株)、Lactococcus(40株)、Enterococcus(89株)。其中,Enterococcus所占比例最大,达到46%。【结论】新疆喀什地区母乳中乳酸菌多样性丰富,极具开发潜力,将为开发母乳中的益生菌以及安全可靠的微生态制剂提供理论依据。

[Objective] Assessment the diversity of lactic acid bacteria in the breast milk of healthy women. ]Methods[ The diversity of lactic acid bacteria was analyzed by using pure culture, repetitive genomic fingerprinting （Rep-PCR） analysis patterns and 16S rRNA gene sequence analysis. [Results] A total of 193 strains of lactic acid bacteria were obtained from eleven breast milk samples. The phylogenetic analysis showed that these strains belonged to four phylogenetic groups, they were Lactobacillus, Streptococcus, Lactococcus and Enterococcus respectively. The dominant genus was Enterococcus, which is up to 46%. [Conclusion] The abundant diversity of lactic acid bacteria in the human breast milk of Kashi area in Xinjiang provided rich resources for exploiting probiotic lactic acid bacteria products.