高效液相色谱法测定纺织品中3种短链对特辛基苯酚乙氧基化物

HPLC Determination of 3 Kinds of Short Chain 4-(1,1,3,3-Tetramethylbutyl) phenol,Ethoxylated in Textiles

样品经甲醇超声萃取2次,萃取液浓缩后用异丙醇定容,分析物在Dikama platisil silica硅胶柱(250mm×4.6mm,5.0μm)上分离,以正己烷-异丙醇(85+15)混合液为流动相A、异丙醇-水(85+15)混合液为流动相B进行梯度洗脱。采用荧光检测器检测,激发波长为230nm,发射波长为296nm。3种对特辛基苯酚乙氧基化物的线性范围均为0.50~50mg·L^(-1),检出限(3S/N)在0.30~0.40mg·kg^(-1)之间。加标回收率在94.4%~106%之间,测定值的相对标准偏差(n=6)小于6.0%。

The sample was ultrasonically extracted twice with methanol.The extract was concentrated and then diluted with isopropanol.The analytes were seperated on a Dikama Platisil silica column （250mm×4.6mm,5.0μm）,with N-hexane-isopropanol （85＋15） as mobile phase A,isopropanol-water （85＋15） as mobile phase B for gradient elution.Fluoresence detector was used with the excitation wavelength of 230 nm and the emission wavelength of 296 nm.Linearity ranges of the 3 kinds of 4-（1,1,3,3-tetramethylbutyl）phenol,ethoxylated were all 0.50-50mg·L^-1,with detection limits （3S/N） of 0.30-0.40mg·kg^-1.Recovery rates obtained by standard addition method were in the range of 94.4%-106% and RSDs （n=6） were less than 6.0%.