华中枸骨叶抑菌物质提取工艺优化及其抑菌作用研究

Optimizing process of extraction and antibacterial activities of antibacterial substance from Ilex centrochinensis S.Y.Hu leaf

本文以抑菌圈直径为指标,以金黄色葡萄球菌和大肠埃希菌为指示菌,比较煎煮法、超声提取、回流提取三种方法所得提取物的抑菌效果,确定最佳的提取方法。在此基础上通过单因素和正交实验优化该方法的提取工艺,最终测定优化工艺条件下的提取物对金黄色葡萄球菌、大肠埃希菌、产气肠杆菌、铜绿假单胞菌、宋内志贺菌、白假丝酵母菌的抑菌效果。结果表明:最佳提取方法为超声波法水提,优化的工艺条件:在超声功率250 W,室温下提取,料液比1∶25(g∶m L),每次30 min,提取2次,此条件下的提取物(原液)对金黄色葡萄球菌、大肠埃希菌的抑菌圈直径分别为24.67、15.67 mm,对产气肠杆菌、铜绿假单胞菌、宋内志贺菌的抑菌圈直径为13.67~17.33 mm,对白假丝酵母菌无抑菌作用。

The size of inhibition zone was the index,S. aureus and E. coli were the indicator bacteria,to determine the best extraction method by comparing the bacteriostatic effect of extracts from water extraction,ultrasonic extraction,reflux extraction.Basis on the single-factor experiment,orthogonal experiment was used to optimize the extraction process.The effect of extracts from the optimal process on S.aureus,E.coli,E.aerogenes,P.aeruginosa,S.sonnei,C.albicans was determined.The results showed that the best extraction process was the use of water as solvent,ultrasound extraction,ultrasonic power 250 W,at roomtemperature,material liquid ratio of 1∶ 25,extraction for 30 minutes,extracted 2 times. Under these condition of extraction,the diameter of bacteriostatic rings against S.aureus,E.coli were 24.67 mm and 15.67 mm,and the diameter of bacteriostatic rings against E.aerogenes,P.aeruginosa,S.sonnei were 13.67~ 17.33 mm,and the extraction had no inhibitory effect on C.albicans.