藜麦麸皮总皂苷的提取纯化工艺研究

Extraction and purification of total saponins from quinoa bran

采用溶剂回流提取法对藜麦麸皮中的总皂苷进行提取,以响应面实验对提取工艺进行优化。以吸附率和解析率为指标,筛选适合用于藜麦麸皮总皂苷纯化的大孔吸附树脂,并对纯化工艺进行优化。优化获得的提取工艺为:料液比1∶20.8,以72%乙醇在72℃提取147 min,皂苷得率可达到1.685%。经过比较,D101大孔吸附树脂的吸附率和解析率都高于HPD600、S-8和AB-8,故选其用作纯化研究,结果表明上样浓度为4 mg/m L,上样液p H6,上样流速为2 BV/h时吸附率最大;用90%乙醇,以2 BV/h的流速洗脱时,解析率最大,经浓缩和冻干所得的皂苷,其纯度达到43.6%。该研究结果为藜麦麸皮的高值利用及藜麦总皂苷生物活性研究和开发利用奠定了基础。

Solvent reflux method was used for saponins extraction and the responsive surface methodology was used to optimize the related parameters.Adsorption rate and desorption rate were used as indicators for screening of proper resin for saponins purification and determining of purification techniques. The obtained optimal extraction technique with a ratio（ bran to 72%ethanol） of 1∶ 20.8,carried out at 72 ℃ for 147 min,and the obtaining rate of saponins reached 1.685%. Compared with HPD600,S-8 and AB-8,the resin D101 showed the highest absorption rate and the highest desorption rate,so it was used for later purification studies. The results suggested that the highest absorption rate for D101 was obtained with a sample concentration of 4 mg/m L,the loading sample was adjusted to p H6 and loading at a velocity of 2 BV/h.The highest desorption rate was obtained when elute with 90% ethanol at a speed of 2 BV/h. The desorption fraction was condensed and freezing dried,the purity of resulted saponins powder reached 43.6%. The above results will facilitate the high-value application of quinoa bran and the related research work of quinoa saponins.