摘要
以‘凤丹’及紫斑牡丹种胚为外植体,通过研究激素、光照条件、培养基成分及活性炭(AC)等对离体胚培养的影响,建立了牡丹胚培养直接成苗的高效、快捷的方法。主要结果:在改良MS(钙加倍)+0.2 g·L^(-1)AC+0.5 mg·L^(-1)BA+0.5 mg·L^(-1)GA中离体胚10 d胚根萌动,20 d长出真叶,25 d根系发达,40 d移栽,3个月后幼苗长势良好。实验得出适宜‘凤丹’的培养基:改良MS(钙加倍、大量元素加倍)+0.6 g·L^(-1)AC+1.0 mg·L^(-1)GA,紫斑牡丹:改良MS(钙加倍、大量元素加倍)+0.6 g·L^(-1)AC+0.5 mg·L^(-1)GA;其中‘凤丹’的成苗率63.88%,成活率66.34%,紫斑成苗率93.75%,成活率在品种间有差异,最佳可达100%。
This study used Paeonia ostii ‘Fengdan ' and Paeonia rockii as explants,established efficient method of Seedling-raising of tree peony embryo culture through the study of hormones,light conditions,medium ingredients and activated carbon( AC) on the in vitro embryo culture. The main result: In vitro embryos radicle germination in 10 d,grow true leaves in 20 d,root system developed in 25 d,transplanting in 40 d,after 3 months the seedlings grow well in the medium of improved MS( calcium doubled) + 0. 2 g·L^(-1)AC +0. 5 mg · L^(-1)GA + 0. 5 mg · L^(-1)BA. Suitable medium for ‘Fengdan ': improved MS( calcium doubled,macroelement doubled) + 0. 6 g · L^(-1)AC + 1. 0 mg · L^(-1)GA; medium for P. rockii: improved MS( calcium doubled,macroelement doubled) + 0. 6 g·L^(-1)AC + 0. 5 mg·L^(-1)GA; The seedling rate of ‘Fengdan' was 63. 88%,the survival rate was 66. 34%,and the rate of seedling of P. rockii was 93. 75%,and survival rates vary among breeds,with a maximum of 100%.
出处
《植物研究》
CAS
CSCD
北大核心
2017年第5期690-699,共10页
Bulletin of Botanical Research
基金
北京市支持中央在京高校共建项目(2016GJ-02)~~
关键词
胚培养
'凤丹’
紫斑牡丹
活性炭
embryo culture
Paeonia ostii 'Fengdan'
Paeonia rockii
activated carbon