牡丹胚培养快速成苗技术研究

Study on Rapid Seedling-raising Technology of Tree Peony Embryo Culture

以‘凤丹’及紫斑牡丹种胚为外植体,通过研究激素、光照条件、培养基成分及活性炭(AC)等对离体胚培养的影响,建立了牡丹胚培养直接成苗的高效、快捷的方法。主要结果:在改良MS(钙加倍)+0.2 g·L^(-1)AC+0.5 mg·L^(-1)BA+0.5 mg·L^(-1)GA中离体胚10 d胚根萌动,20 d长出真叶,25 d根系发达,40 d移栽,3个月后幼苗长势良好。实验得出适宜‘凤丹’的培养基:改良MS(钙加倍、大量元素加倍)+0.6 g·L^(-1)AC+1.0 mg·L^(-1)GA,紫斑牡丹:改良MS(钙加倍、大量元素加倍)+0.6 g·L^(-1)AC+0.5 mg·L^(-1)GA;其中‘凤丹’的成苗率63.88%,成活率66.34%,紫斑成苗率93.75%,成活率在品种间有差异,最佳可达100%。

This study used Paeonia ostii ‘Fengdan ＇ and Paeonia rockii as explants,established efficient method of Seedling-raising of tree peony embryo culture through the study of hormones,light conditions,medium ingredients and activated carbon（ AC） on the in vitro embryo culture. The main result： In vitro embryos radicle germination in 10 d,grow true leaves in 20 d,root system developed in 25 d,transplanting in 40 d,after 3 months the seedlings grow well in the medium of improved MS（ calcium doubled） ＋ 0. 2 g·L^（-1）AC ＋0. 5 mg · L^（-1）GA ＋ 0. 5 mg · L^（-1）BA. Suitable medium for ‘Fengdan ＇： improved MS（ calcium doubled,macroelement doubled） ＋ 0. 6 g · L^（-1）AC ＋ 1. 0 mg · L^（-1）GA; medium for P. rockii： improved MS（ calcium doubled,macroelement doubled） ＋ 0. 6 g·L^（-1）AC ＋ 0. 5 mg·L^（-1）GA; The seedling rate of ‘Fengdan＇ was 63. 88%,the survival rate was 66. 34%,and the rate of seedling of P. rockii was 93. 75%,and survival rates vary among breeds,with a maximum of 100%.