环己胺的微生物代谢及相关氧化酶的研究进展

Biodegradation of cyclohexylamine by microbes and related cyclohexylamine oxidase: a review of recent studies

环己胺氧化酶属于一种胺氧化酶,目前仅对动植物体内的胺氧化酶进行了系统研究,但对于不同微生物来源的环己胺氧化酶了解不够广泛。在微生物代谢途径中,参与上游代谢的基因具有丰富的多样性,而参与下游代谢途径的基因模块在不同的微生物中相对保守。因此,研究微生物代谢环己胺的关键是探讨催化环己胺生成环己酮的环己胺氧化酶的催化机制。该文综述了目前国内外微生物降解环己胺的研究进展,介绍了环己酮单加氧酶和环己胺氧化酶在有机胺的微生物代谢途径研究中的作用,并对其潜在应用前景进行了展望。

Cyclohexylamine oxidase is one of the amine oxidases. Currently, researchers only studied on the animals and plants amine oxidase, but the study of cyclohexylamine oxidase from different microbial sources is not enough. The genes in upstream metabolism in the microbial metabolic pathways are diversified and the downstream metabolism pathways are conserved in different microorganisms. Therefore, the key to study metabolic mechanism of cyclohexylamine microbial metabolism is to reveal the catalytic mechanism of cyclohexylamine oxidase catalyzed cyclohexylamine to cyclohexanone. The present paper summarized the current research progress of microbial degradation of cyclohexylamine. The importance of cyclohexanone monooxygenase and cyclohexylamine oxidase in the study of microbial metabolic pathway of organic amines was also introduced and its potential application was prospected.