肉鸡腹脂双向选择系中RNA编辑位点的鉴定研究

Identification of RNA Editing Sites in Chicken Lines Divergently Selected for Abdominal Fat Content

旨在准确获得肉鸡腹脂双向选择系脂肪组织中的RNA编辑位点信息,进而为脂肪相关研究提供更多的信息来源。本研究以东北农业大学肉鸡高、低腹脂双向选择品系第十九世代肉鸡为试验材料,使用转录组测序(RNA-seq)和基因组测序数据识别肉鸡腹部脂肪组织RNA编辑位点。通过生物信息学手段,设定严格的数据过滤与筛选策略,构建RNA编辑位点候选集。结果表明,在候选集中,通过对RNA编辑位点的分类和注释,在低脂系肉鸡脂肪组织中发掘转换型RNA编辑位点28个,颠换型RNA编辑位点101个,插入缺失型RNA编辑位点71个;在高脂系肉鸡脂肪组织中,发掘转换型RNA编辑位点30个,颠换型RNA编辑位点84个,插入缺失型RNA编辑位点77个。绝大多数RNA编辑位点落在内含子、基因间区域及基因上游区域(内含子区41.91%,基因间区域33.08%,基因下游20.59%,基因上游2.94%和外显子区1.47%)。试验验证了4个候选RNA编辑位点,其中3个位点落在与脂肪形成有重要关系的ATP1B3、SLC6A6和FLNB基因区域。本研究鉴定和验证了存在于肉鸡脂肪组织中的RNA编辑位点,为进一步研究RNA编辑影响脂肪组织生长发育的分子机制奠定了基础。

This study aimed to obtain the RNA editing sites information in adipose tissue in chicken lines divergently selected for abdominal fat content, and provide the foundation for fat study of broiler. Ten individuals from the 19th generation of fat and lean broiler lines divergently selected for abdominal fat content from the Northeast Agricultural University were used. The transcrip- tome and genome sequencing datasets were used for identifying RNA editing sites tissues. Bioinformatics method was used to construct the candidate set stringent data filtering and screening. The result showed that we cla of RNA ed ssified and in abdominal fat iting sites, after annotated RNA editing sites in the candidate set. In the adipose tissue of lean line broilers, 28 transition, 101 transversion and 71 insertion-deletion （InDel） RNA editing sites types were founds while in the adipose tissue of fat line broilers, 30 transition, 84 transversion and 77 InDel RNA editing sites types were found. Most of RNA editing sites were located in the regulatory and intergenic regions of genes （intron 41.91~ intergenic 33.08~ downstream 20.59~/0 ~ upstream 2.94~ and exon 1.47 ~, respectively）. We validated 4 RNA editing sites, 3 of which located in genes （ATP1B3, SLC6A6 and FLNB） related to chicken adipogenesis. In the current study, identification and validation of RNA editing sites were performed in chicken abdominal fat tissues, which lays the foundation for further investigating the molecular mechanism of RNA editing in adipose tissue growth and development.