辅酶Q10中枢神经系统的安全性评价

Safety evaluation of coenzyme Q10 on central nervous system

目的考察大剂量ig辅酶Q10对小鼠中枢神经系统的影响,为临床安全性评价提供实验依据。方法将小鼠随机分为溶媒对照组、紫苏油对照组、阳性对照组(氯丙嗪或地西泮)和辅酶Q10低、中、高剂量组(1.5、3.0和6.0 g/kg,相当于临床等效剂量的75、150和300倍),每组12只,雌雄各半,40 m L/kg单次ig给药。直接观察小鼠一般行为活动;转棒法观察小鼠运动协调能力;Anymaze动物行为学视频分析系统观察小鼠自发活动和与阈下剂量戊巴比妥钠的协同作用。结果与溶媒对照组、紫苏油对照组比较,辅酶Q10 3个剂量组对小鼠一般行为活动、自发活动、运动协调能力和与阈下剂量戊巴比妥钠的协同作用均无显著差异。结论大剂量ig辅酶Q10对小鼠中枢神经系统未见明显毒性作用。

Objective To investigate the effect of high-dose coenzyme Q10 on the central nervous system in mice, and to provide experimental basis for clinical safety evaluation. Methods Mice were randomly divided into vehicle control group, perillartine control group, positive control group （chlorpromazine or diazepam） and coenzyme Q10 low, medium and high dose groups （1.5, 3.0 and 6.0 g/kg, equivalent to 75, 150, and 300 times of clinical dosage, respectively）. The corresponding drugs were ig given to mice with the volume of 40 mL/kg. The general behavior of mice was observed directly, the motor coordination ability was observed by rotating stick method, and Anymaze animal behavior video analysis system was used to observe the spontaneous activity of mice and synergistic reaction with sub-threshold dose of pentobarbital sodium. Results There were no significant differences in the general behavioral activity, and the number of spontaneous activity times, mean resident time, and ratio of sleeping were found in all coenzyme Q10 groups, compared with the vehicle and perillartine control groups. Conclusion High dose of coenzyme Q10 has no significantly toxic effect on the central nervous system in mice, which could provide a reliable experimental basis for further medication study and clinical application of high-dose coenzyme Q10.