异烟肼对大鼠免疫功能影响的研究

Influence of isoniazid on immune function of rat

目的研究异烟肼对大鼠淋巴细胞因子表达和巨噬细胞功能的影响。方法健康雄性SD大鼠,随机分为喂药1个月组、喂药3个月组、停药1个月(喂药3个月后)组,每组再随机等分为异烟肼组和对照组。异烟肼组给以120 mg/kg异烟肼,隔日1次ig给药,对照组给予生理盐水。于相应时间点,ELISA法检测血清白细胞介素-12(IL-12)、干扰素-γ(IFN-γ)水平;琼脂平板法检测大鼠血清溶菌酶含量;硝酸铅(Comori)法检测腹腔液酸性磷酸酶水平。结果在各时间点,异烟肼组IL-12、IFN-γ、溶菌酶水平与对照组比较,差异均不显著;喂药1个月,与对照组比较,异烟肼组酸性磷酸酶水平显著升高(P<0.05);喂药3个月、停药1个月,两组酸性磷酸酶水平差异不显著。结论 120 mg/kg异烟肼对大鼠免疫功能无明显影响,长期应用不会导致免疫功能的损伤。

Objective To study the influence of isoniazid on lymphocyte factor expression and macrophage function of rats. Methods Healthy male SD rats were randomly divided into three groups, which was treated for one month, three months and withdrawal for one month after treated for three months, and each group was randomly divided into isoniazid group and control group. The isoniazid groups were ig with isoniazid at dose of 120 mg/kg every other day and control groups were fed on normal saline. At the corresponding time points, the level of interleukin-12 （IL-12） and interferon-3, （IFN-3,） was detected with ELISA method, detected serum lysozyme content by agar plate method, and Comori method was used for the detection of acid phosphatase levels in peritoneal fluid. Results At all the time points, levels of IL-12, IFN-3, and lysozyme in isoniazid group were not significantly different compared with control group. There were statistically significant differences in acid phosphatase between isoniazid group and control group after treated for one month （P 〈 0.05）, but the significant differences disappeared at the next two time points. Conclusion Isoniazid of 120 mg/kg may have no obvious influence on the immune function of rat. We don＇t detect the immune injury.