复荣通脉胶囊对糖尿病大鼠心肌Smad7蛋白表达的影响

Effect of Furong Tongmai Capsules on Smad7 expression in myocardium of rats with diabetes mellitus

目的探讨复荣通脉胶囊对糖尿病大鼠心肌Smad7蛋白表达的影响。方法采用随机数字表法将60只大鼠分为正常组(10只)、造模组(50只)。成模后,将40只糖尿病大鼠随机分为糖尿病组和复荣通脉低、中、高剂量组,每组各10只。复荣通脉低、中、高剂量组分别给予复荣通脉胶囊0.7、1.4、2.8 g/kg灌胃,正常组和糖尿病组给予等量纯净水灌胃。8周后,留取大鼠心肌制备病理切片,观察各组血糖、血脂及Smad7蛋白的阳性表达。结果治疗前、治疗8周后,糖尿病组和复荣通脉低、中、高剂量组血糖水平均明显高于正常组(P<0.05),各组大鼠组内治疗前后血糖差异无统计学意义(P>0.05)。治疗8周后,糖尿病组和复荣通脉低、中、高剂量组总胆固醇、三酰甘油水平均明显高于正常组(P<0.05)。正常组Smad7蛋白表达呈强阳性,糖尿病组和复荣通脉低、中、高剂量组Smad7蛋白表达均明显下降(P<0.05﹚;与糖尿病组、复荣通脉低剂量组比较,复荣通脉中、高剂量组Smad7蛋白高表达(P<0.05﹚。结论复荣通脉胶囊具有上调糖尿病大鼠心肌Smad7蛋白表达、抑制糖尿病心肌细胞损伤的作用。

Objective To investigate the expression of Furong Tongmai Capsules on Smad7 expression in myocardium of rats with diabetes mellitus. Methods Sixty rats were randomly divided into normal group （10 rats） and model group （50 rats） by random number table method. After the model was made, 40 rats with diabetes mellitus were randomly divided into diabetes mellitus group and low, medium, high dose group of Furong Tongmai Capsules, with 10 rats in each group. Low, medium, high dose group of Furong Tongmai Capsules were given 0.7, 1.4, 2.8 g/kg of Furong Tongmai Capsules by gavage, normal group and diabetes mellitus group were given equal quantity of purified water. After 8 weeks, the myocardium in rats was preserved to make pathological section. The positive expression of blood glucose, blood lipid, Smad7 protein in each group was observed. Results Before treatment and after treatment for 8 weeks, the levels of blood glucose in diabetes mellitus group and low, medium, high dose group of Furong Tongmai Capsules were all higher than those of normal group （P 〈 0.05）, while there were no significant differences of blood glucose within each group before and after treatment （P 〉 0.05）. After treatment for 8 weeks, the levels of total cholesterol, triacylglycerol in diabetes mellitus group and low, medium, high dose group of Furong Tongmai Capsules were all higher than those of normal group （P 〈 0.05）. The Smad7 of normal group showed high positive expression, which of diabetes mellitus group and lowl medium, high dose group of Furong Tongmai Capsules was all decreased （P 〈 0.05）; compared with diabetes mellitus group and low dose group of Furong Tongmai Capsules, the Smad7 expression of medium, high dose group Of Furong Tongmai Capsules was increased significantly （P 〈 0.05）. Conclusion Furong Tongmai Capsules have the function of increasing the Smad7 expression in myocardium of rats with diabetes mellitus, inhibiting myocardial cell injury.