淫羊藿多糖对卵泡细胞凋亡的作用及机理研究

Effects and Mechanisms of Epimedium Polysaccharides on Murine Follicular Cell Apoptosis

目的:研究淫羊藿多糖对卵泡细胞凋亡的作用及机理。方法:对4周龄健康雌性小鼠连续灌服不同剂量淫羊藿多糖7 d,并采集各组小鼠卵巢卵泡细胞,检测卵泡细胞凋亡情况,RT-PCR检测凋亡相关基因Bcl-2/Bax表达水平;同时完整分离心脏、肝脏、脾脏、肺脏和肾脏,称重并计算各组内脏指数。结果:8、6 mg组和生理盐水对照组的卵泡细胞总凋亡率分别为10.89%、8.75%和11.74%;RT-PCR技术测定Bcl-2和Bax凋亡相关基因的表达发现,抑制凋亡基因Bcl-2 mRNA的表达显著升高。结论:淫羊藿多糖可有效抑制小鼠卵巢卵泡细胞凋亡,这一作用主要与调节细胞凋亡的Bcl-2/Bax基因表达有关。

Objective： To investigate the effect and mechanism of Epimedium polysaccharides on apoptosis in follicular cells. Methods： After 4 week old healthy female mice with different dose of Epimedium Polysaccharide for seven days,ovarian follicular cells of mice were collected,with follicular cell apoptosis,RT-PCR detection of apoptosis related gene Bcl-2/Bax expression level; at the same time the complete separation of the heart,liver,spleen,lung and kidney,weight and Viscera index were calculated. Results： Group 8,6 mg and saline control group of follicular cells apoptosis rate were 10. 89 %,8. 75 % and 11. 74 % respectively; the determination of the expression of Bcl-2 and apoptosis related gene Bax,RT-PCR technology,the expression of apoptosis inhibiting gene Bcl-2 mRNA were significantly increased. Conclusion： Epimedium Polysaccharide can effectively inhibit the apoptosis of ovarian follicular cells in mice,which is mainly related to the expression of Bcl-2/Bax gene regulating apoptosis.