核转录因子κB相关蛋白在特发性眼眶炎性假瘤细胞中的表达变化及其意义

Expression and significance of nuclear factor-KB-related proteins in idiopathic orbital inflammatory pseudotumor

背景特发性眼眶炎性假瘤（IOIP）是常见的眼眶疾病之一，症状严重，治疗后易复发，其发病机制尚不清楚。研究表明，核转录因子κB（NF-κB）相关蛋白参与炎症反应、免疫应答以及细胞的重要病理生理过程，其在IOIP的发生过程中是否发挥作用值得关注。目的探讨NF-κB信号通路在IOIP发病机制中的作用。方法于2010年9月至2016年5月收集在北京同仁医院眼科手术切除并经组织病理学证实的IOIP组织标本24例，制备石蜡切片，采用苏木精一伊红染色法检查IOIP的组织病理学特征，采用免疫组织化学法检测和定位IOIP细胞中NF-κB相关蛋白NF-κB／p65、p-p65、p50和κB抑制蛋白α（IκB-α）的表达，采用免疫细胞化学法及Westernblot法对免疫组织化学检测结果进行验证。结果IOIP的病理组织学特征为以小淋巴细胞为主的多种炎性细胞浸润及不同程度的纤维结缔组织增生，病灶涉及泪腺时可见大量上皮样细胞。免疫组织化学法检测显示，24例IOIP标本的细胞质中均可见NF-κB／p65阳性表达，其中15例可见细胞核中NF-κB／p65呈阳性表达，占62．5％；22例IOIP标本的细胞质中p50呈阳性表达，占91．7％，其中17例细胞核中p50呈阳性表达，占70．8％；22例IOIP标本中p-p65呈阳性表达，占91．7％；11例IOIP标本中IκB-α呈阳性表达，均定位于细胞质，占45．8％。免疫细胞化学法及Westernblot结果与免疫组织化学法检测结果一致。结论IOIP发病过程中NF-κB信号通路可能被激活，NF-κB信号通路可能参与IOIP的发病机制。

Background Idiopathic orbital inflammatory pseudotumor （IOIP） is a commom orbital disease, with serious eye symptoms and replase tendency, and its pathogenesis is still unclear. Nuclear factor-κB （NF-κB） - related proteins participate in many important pathophysiological process,however,whether NF-κB plays a role in the IOIP process is worthy of attention. Objective This study was to explore the roles of NF-κB pathway in IOIP pathogenesis. Methods Twenty-four IOIP specimens were collected during surgery in Beijing Tongren Hospital from September 2010 to May 2016. The histopathological characteristics of IOIP were examined by hematoxylin and eosin staining. The expression and location of NF-κB/p65 ,p-p65 ,p50 and inhibitor of κB （IκB-α） were detected by immunohistochemistry and verified by immunocytochemistry and Western blot assay. Results The histopathological features of IOIP were numerous small lymphocyte infiltraion and fibrous tissue proliferation, and a lot of epithelioid cells were seen in lacrimal gland-involved specimens. NF-κB/p65 was positively expressed in the cytoplasm of all 24 specimens and the nucleus in 15 specimens with the expressing rate of 62.5%. p50 was expressed in the cytoplasm in 22 specimens with the expressing rate of 91.7% and in 70. 8%. The positive expression of p-p65 was found in 22 the nucleus in 17 specimens with the expressing rate of specimens with the expressing rate of 91.7% , and IκB-α was expressed in the cytoplasm of 11 specimens with the expressing rate of 45.8%. These results were confirmed by immunocytochemistry and Western blot assay. Conclusions NF-κB pathway is activiated during IOIP process,and NF-κB pathway may be involved in the pathogenesis of IOIP.